Silver fir (Abies alba L.) polyphenolic extract shows beneficial influence on chondrogenesis in vitro under normal and inflammatory conditions

Sirše, Mateja (author), Fokter, Samo K. (author), Štrukelj, Borut (author), Zupan, Janja (author)

Several plant polyphenols have been shown to reduce osteoarthritis symptoms due to their antioxidant, anti-inflammatory and immunomodulatory properties. We investigated the effects of two different polyphenolic extracts (Belinal, Pycnogenol) and two different polyphenols (resveratrol, quercetin) on the chondrogenic potential of bone-derived mesenchymal stem/stromal cells (MSCs) from healthy donors and patients with osteoarthritis. Our main aim was to determine whether Belinal, a commercially available polyphenolic extract from silver fir (Abies alba L.) branches, has comparable chondrogenic potential with the other tested extract and the polyphenols under inflammatory and non-inflammatory conditions. In our study, Belinal promoted significantly greater chondrogenesis compared to the untreated (p = 0.0289) and resveratrol-treated (p = 0.0468) MSCs from patients with hip osteoarthritis under non-inflammatory conditions. Under inflammatory conditions, chondrogenesis was significantly enhanced for MSCs treated with Belinal compared to the control (p = 0.0483). The other extract and the polyphenols did not show any significant effects on chondrogenesis under non-inflammatory or inflammatory conditions. None of the tested extracts and polyphenols showed significant effects on chondrogenesis in healthy donors, under either non-inflammatory or inflammatory conditions. Our data show that Belinal can boost the chondrogenesis of MSCs derived from patients with osteoarthritis, under both non-inflammatory and inflammatory conditions.

• Language: English
• Keywords: mesenchymal stem cells, mesenchymal stromal cells, polyphenols, chondrogenesis, inflammation, subchondral bone, osteoarthritis
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 14 str.
• Numbering: Vol. 25, iss. 20, art. 4616
• PID: 20.500.12556/RUL-134462
• UDC: 616.72-002
• ISSN on article: 1420-3049
• DOI: 10.3390/molecules25204616
• COBISS.SI-ID: 32142339
• Publication date in RUL: 17.01.2022

Record is a part of a journal

• Title: Molecules
• Shortened title: Molecules
• Publisher: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 11.10.2020

Secondary language

• Language: Slovenian
• Keywords: polifenoli, stromalne celice, hondrogeneza, vnetje, osteoartritis, mezenhimske matične celice

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: J3-1749
• Name: Mezenhimske matične celice – nosilci endogene regenerativne sposobnosti tkiv v boju proti staranju mišično-skeletnega sistema

• Funder: Other - Other funder or multiple funders
• Funding programme: University Medical Centre Maribor
• Project number: IRP-2019/01-09