Structural isomerism and enhanced lipophilicity of pyrithione ligands of organoruthenium(II) complexes increase inhibition on AChE and BuChE

Kladnik, Jerneja (author), Ristovski, Samuel (author), Kljun, Jakob (author), Defant, Andrea (author), Mancini, Ines (author), Sepčić, Kristina (author), Turel, Iztok (author)

The increasing number of Alzheimer’s disease (AD) cases requires the development of new improved drug candidates, possessing the ability of more efficient treatment as well as less unwanted side effects. Cholinesterase enzymes are highly associated with the development of AD and thus represent important druggable targets. Therefore, we have synthesized eight organoruthenium(II) chlorido complexes 1a–h with pyrithione-type ligands (pyrithione = 1-hydroxypyridine-2(1H)-thione, a), bearing either pyrithione a, its methyl (b-e) or bicyclic aromatic analogues (f–h) and tested them for their inhibition towards electric eel acetylcholinesterase (eeAChE) and horse serum butyrylcholinesterase (hsBuChE). The experimental results have shown that the novel complex 1g with the ligand 1-hydroxyquinoline-2-(1H)-thione (g) improves the inhibition towards eeAChE (IC$_{50}$ = 4.9 µM) and even more potently towards hsBuChE (IC$_{50}$ = 0.2 µM) in comparison with the referenced 1a. Moreover, computational studies on Torpedo californica AChE have supported the experimental outcomes for 1g, possessing the lowest energy value among all tested complexes and have also predicted several interactions of 1g with the target protein. Consequently, we have shown that the aromatic ring extension of the ligand a, though only at the appropriate position, is a viable strategy to enhance the activity against cholinesterases.

• Language: English
• Keywords: Alzheimer’s disease, acetylcholinesterase, butyrylcholinesterase, cholinesterase inhibitor, molecular docking, organoruthenium(II) complexes, pyrithione derivatives
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology; BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 17 str.
• Numbering: Vol. 21, iss. 16, art. 5628
• PID: 20.500.12556/RUL-134383
• UDC: 546.96
• ISSN on article: 1661-6596
• DOI: 10.3390/ijms21165628
• COBISS.SI-ID: 24569091
• Publication date in RUL: 12.01.2022

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1661-6596
• COBISS.SI-ID: 36217605

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 06.08.2020

Secondary language

• Language: Slovenian
• Keywords: rutenij, organorutenijevi kompleksi, ligandi, derivati piritiona, Alzheimerjeva bolezen