Alteration of the phenylpropanoid pathway by watercore disorder in apple (Malus x domestica)

Čebulj, Anka (avtor), Mikulič Petkovšek, Maja (avtor), Rares Lucaciu, Calin (avtor), Veberič, Robert (avtor), Marinovic, Silvija (avtor), Kolarek, Martina (avtor), Hutabarat, Olly Sanny (avtor), Faramarzi, Shabad (avtor), Rattei, Thomas (avtor), Molitor, Christian (avtor), Hudina, Metka (avtor), Haselmair-Gosch, Christian (avtor), Halbwirth, Heidi (avtor), Slatnar, Ana (avtor)

Watercore is a physiological disorder of apple expressed as fluid deposition in intercellular spaces. We analysed the watercore prone cultivar 'Fuji' to establish a link between enzymatic response and fluid accumulation in the fruit. Individual phenolic content, selected enzyme activities of the phenylpropanoid pathway, sugars and organic acids were quantified in watercore affected flesh and peel. In addition, transcriptome sequencing was performed to obtain a first insight into molecular mechanisms underlying the physiological disorder. Sampled material included: peel (HP) and flesh from healthy fruit (H), peel from watercore affected fruit (WP), healthy flesh from fruit with watercore (HW) and watercore flesh (WW) from fruit with watercore. A sorbitol increase in watercore affected apple was observed not only in the flesh but also in the peel. Moreover, two phenolic groups (hydroxycinnamic acids and flavonols) were significantly affected by watercore and their content was higher in WP in comparison to HP. Dihydrochalcone content was the highest in WW flesh. This was supported by RNA-seq data, which indicated a generally increased expression of genes from the early flavonoid pathway, sorbitol metabolism and polyphenol oxidases. Among the phenylpropanoid pathway enzymes analysed, phloretin 2′-O-glycosyltransferase was the only one exhibiting altered activity.

• Jezik: Angleški jezik
• Ključne besede: dihydrochalcones, enzyme activity, gene expression, flavonoid pathway, phenolic compounds, physiological disorder
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Recenzirani rokopis
• Datum objave: 31.07.2021
• Leto izida: 2021
• Št. strani: 9 str.
• Številčenje: Vol. 289, art. 110438
• PID: 20.500.12556/RUL-134368
• UDK: 634
• ISSN pri članku: 1879-1018
• COBISS.SI-ID: 72043779
• Datum objave v RUL: 11.01.2022

Gradivo je del revije

• Naslov: Scientia horticulturae
• Založnik: Elsevier
• ISSN: 1879-1018
• COBISS.SI-ID: 23111173

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.
• Začetek licenciranja: 31.07.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: jabolka, dihidrohalkoni, aktivnost encimov, izražanje genov, sintezna pot flavonoidov, fenolne spojine, fiziološke motnje, Malus x domestica