Hanseniaspora smithiae sp. nov., a novel apiculate yeast species from Patagonian Forests that lacks the typical genomic domestication signatures for fermentative environments

Čadež, Neža (author), Bellora, Nicolas (author), Ulloa, Ricardo (author), Tome, Miha (author), Petković, Hrvoje (author), Groenewald, Marizeth (author), Hittinger, Chris Todd (author), Libkind, Diego (author)

During a survey of Nothofagus trees and their parasitic fungi in Andean Patagonia (Argentina), genetically distinct strains of Hanseniaspora were obtained from the sugar-containing stromata of parasitic Cyttaria spp. Phylogenetic analyses based on the single-gene sequences (encoding rRNA and actin) or on conserved, single-copy, orthologous genes from genome sequence assemblies revealed that these strains represent a new species closely related to Hanseniaspora valbyensis. Additionally, delimitation of this novel species was supported by genetic distance calculations using overall genome relatedness indices (OGRI) between the novel taxon and its closest relatives. To better understand the mode of speciation in Hanseniaspora, we examined genes that were retained or lost in the novel species in comparison to its closest relatives. These analyses show that, during diversification, this novel species and its closest relatives, H. valbyensis and Hanseniaspora jakobsenii, lost mitochondrial and other genes involved in the generation of precursor metabolites and energy, which could explain their slower growth and higher ethanol yields under aerobic conditions. Similarly, Hanseniaspora mollemarum lost the ability to sporulate, along with genes that are involved in meiosis and mating. Based on these findings, a formal description of the novel yeast species Hanseniaspora smithiae sp. nov. is proposed, with CRUB 1602$^H$ as the holotype.

• Language: English
• Keywords: apiculate yeast, new species, phylogenomics, OGRI, gene loss, speciation, domestication
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 11 str.
• Numbering: Vol. 12, art. 679894
• PID: 20.500.12556/RUL-134067
• UDC: 579.8:582.282.23
• ISSN on article: 1664-302X
• DOI: 10.3389/fmicb.2021.679894
• COBISS.SI-ID: 72059139
• Publication date in RUL: 23.12.2021

Record is a part of a journal

• Title: Frontiers in microbiology
• Shortened title: Front. microbiol.
• Publisher: Frontiers Research Foundation
• ISSN: 1664-302X
• COBISS.SI-ID: 4146296

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: kvasovke, Hanseniaspora, taksonomija