Intracellular hydrolysis of small-molecule O-linked N-acetylglucosamine transferase inhibitors differs among cells and is not required for its inhibition

Loi, Elena Maria (avtor), Weiss, Matjaž (avtor), Pajk, Stane (avtor), Gobec, Martina (avtor), Tomašič, Tihomir (avtor), Pieters, Roland J. (avtor), Anderluh, Marko (avtor)

O-GlcNAcylation is an essential post-translational modification that occurs on nuclear and cytoplasmic proteins, regulating their function in response to cellular stress and altered nutrient availability. O-GlcNAc transferase (OGT) is the enzyme that catalyzes this reaction and represents a potential therapeutic target, whose biological role is still not fully understood. To support this research field, a series of cell-permeable, low-nanomolar OGT inhibitors were recently reported. In this study, we resynthesized the most potent OGT inhibitor of the library, OSMI-4, and we used it to investigate OGT inhibition in different human cell lines. The compound features an ethyl ester moiety that is supposed to be cleaved by carboxylesterases to generate its active metabolite. Our LC-HRMS analysis of the cell lysates shows that this is not always the case and that, even in the cell lines where hydrolysis does not occur, OGT activity is inhibited.

• Jezik: Angleški jezik
• Ključne besede: ester hydrolysis, inhibitor, O-GlcNAc transferase, OGT inhibitor
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 10 str.
• Številčenje: Vol. 25, iss. 15, art. 3381
• PID: 20.500.12556/RUL-133856
• UDK: 577.121:615
• ISSN pri članku: 1420-3049
• DOI: 10.3390/molecules25153381
• COBISS.SI-ID: 24851459
• Datum objave v RUL: 17.12.2021

Gradivo je del revije

• Naslov: Molecules
• Skrajšan naslov: Molecules
• Založnik: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.08.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: terapevtska uporaba, metaboliti