Escherichia coli isolated from cases of colibacillosis in Russian poultry farms (Perm Krai) : sensitivity to antibiotics and bacteriocins

Kuznetsova, Marina V. (author), Gizatullina, Julia S. (author), Nesterova, Larisa Jurʼevna (author), Starčič Erjavec, Marjanca (author)

Escherichia coli strains isolated from case of colibacillosis in Russian poultry farms in the region of Perm Krai were analyzed for their sensitivity to main antibiotics and bacteriocins. Sensitivity profiles for 9 antibiotics and 20 bacteriocins were determined with the disc diffusion method and the overlay test, respectively. Further, with the PCR the presence of several bla and integron 1 genes was revealed and the phylogenetic group for each strain determined. Among the 28 studied E. coli strains 85.7% were resistant to at least three antibiotics, 53.6% to five or more drugs, and 10.7% to eight antibiotics. PCR revealed that the bla$_{TEM}$ gene was harbored by 71.4% of strains and the bla$_{CTX-M}$ gene by 53.6% of strains. The class 1 integrons were found in 28.6% of strains. All of the studied strains were insensitive to ten or more bacteriocins. More than 90% of the studied strains were insensitive to pore-forming colicins of group A and B colicins, while 60.7% were insensitive to colicins with DNase and RNase activity. All of the analyzed strains were insensitive to at least two of the tested microcins. Neither the antibiotic resistance profile nor the bacteriocin resistance profile correlated with phylogenetic group of the strains. Thus, the studied strains were shown to possess high levels of multiple resistance to antibiotics and insensitivity to bacteriocins.

• Language: English
• Keywords: avian Escherichia coli, antibiotics, bacteriocins, colicins, microcins
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 11 str.
• Numbering: Vol. 8, iss. 5, art. 741
• PID: 20.500.12556/RUL-133718
• UDC: 577
• ISSN on article: 2076-2607
• DOI: 10.3390/microorganisms8050741
• COBISS.SI-ID: 16277507
• Publication date in RUL: 10.12.2021

Record is a part of a journal

• Title: Microorganisms
• Shortened title: Microorganisms
• Publisher: MDPI AG
• ISSN: 2076-2607
• COBISS.SI-ID: 523277081

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 15.05.2020

Secondary language

• Language: Slovenian
• Keywords: ptičja Escherichia coli, antibiotiki, bakteriocini, kolicini, mikrocini