Comparison of selection methods for the establishment of a core collection using SSR markers for hazelnut (Corylus avellana L.) accessions from European germplasm repositories

Boccacci, Paolo (author), Aramini, Maria (author), Ordidge, Matthew (author), Hintum, Theo J. L. van (author), Torello Marinoni, Daniela (author), Valentini, Nadia (author), Sarraquigne, Jean-Paul (author), Solar, Anita (author), Rovira, Merce (author), Bacchetta, Loretta (author), Botta, Roberto (author)

Hazelnut (Corylus avellana L.) is one of the most important tree nut crops in Europe. Germplasm accessions are conserved in ex situ repositories, located in countries where hazelnut production occurs. In this work, we used ten simple sequence repeat (SSR) markers as the basis to establish a core collection representative of the hazelnut genetic diversity conserved in different European collections. A total of 480 accessions were used: 430 from ex situ collections and 50 landraces maintained on-farm. SSR analysis identified 181 genotypes, that represented our whole hazelnut germplasm collection (WHGC). Four approaches (utilizing MSTRAT, Power Core, and Core Hunter%s single- and multi-strategy) based on the maximization (M) strategy were used to determine the best sampling method. Core Hunter%s multi-strategy, optimizing both allele coverage (Cv) and Cavalli-Sforza and Edwards (Dce) distance with equal weight, outperformed the others and was selected as the best approach. The final core collection (Cv-Dce30) comprised 30 entries (16.6% of genotypes). It recovered all SSR alleles and preserved parameter variations when compared to WHGC. Entries represented all six gene pools obtained from the population structure analysis of WHGC, further confirming the representativeness of Cv-Dce30. Our findings contribute towards improving the conservation and management of European hazelnut genetic resources and could be used to optimize future research by identifying a minimum number of accessions on which to focus.

• Language: English
• Keywords: hazelnut, corylus avellana, plant genetic resources, germplasm management, SSR markers, microsatellites, germplasm repositories, in situ conservation, ex situ conservation
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Publication date: 19.11.2021
• Year: 2021
• Number of pages: Str. 1-14 (48)
• Numbering: Vol. 17, Iss. 6
• PID: 20.500.12556/RUL-133415
• UDC: 634.5
• ISSN on article: 1614-2942
• DOI: 10.1007/s11295-021-01526-7
• COBISS.SI-ID: 86288899
• Publication date in RUL: 26.11.2021

Record is a part of a journal

• Title: Tree genetics & genomes
• Shortened title: Tree genet. genomes
• Publisher: Springer.
• ISSN: 1614-2942
• COBISS.SI-ID: 514986265

Secondary language

• Language: Slovenian
• Keywords: leska, corylus avellana, genska banka, mikrosatelitski markerji, genotipi

Projects

• Funder: EC - European Commission
• Name: SAFENUT project (“Safeguard of almond and hazelnut genetic resources: from traditional uses to modern agro-industrial opportunities”), AGRI GEN RES 068