As part of the master's thesis, we examined the effect of salt stress and added putrescine in the nutrient solution on the growth and conversion of polyamines in selected microgreens from the group of legumes, grasses and cruciferous plants. Microgreens were grown under different conditions – in control solution and in solutions with added NaCl and/or putrescine. In the preliminary experiments, we evaluated the influence of salt stress on plant growth and accumulation of polyamines, which were determined chromatographically. Salt stress had a negative effect on plant growth, and we also found differences in susceptibility to salt stress between different species. Based on preliminary experiments, we selected one species from the group of cruciferous plants (garden cress; Lepidum sativum), grasses (wheat; Triticum aestivum) and legumes (alfalfa; Medicago sativa), which were grown under controlled conditions. The addition of putrescine to the nutrient solution had a relatively small effect on the accumulation of polyamines in both the presence and absence of salt stress. Salt stress (150 mM NaCl) resulted in increased spermine content and decreased spermidine content in all three types of microgreens. In alfalfa, salt stress also resulted in lower putrescine and agmatine content in microgreens.
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