Mesenchymal stem cells represent a heterogeneous group of cells that can be isolated from several adult tissues. In recent years the main interest is in their ability to migrate to the site of inflammation in the body, where they secrete various soluble factors such as cytokines, chemokines, and growth factors that improve angiogenesis, inhibit inflammation, and inhibit apoptosis. Their immunomodulatory and regenerative properties are the reason for increasing interest for clinical applications, where they could be used for treatment of many pathological conditions related to inflammation and tissue degeneration. Increasing use requires better cell separation techniques, which need to be faster and more efficient. The purpose of our research was to investigate potential materials which would be suitable for mesenchymal stem cell separation from other cells. We used bone marrow derived human mesenchymal stem. In the first part of the research, we characterized sample materials via measuring the zeta potential, contact angle and roughness. In the second part, we determined the influence of the material properties on the behavior of cells - on their adhesion. The best results were shown on the fibronectin coated microtiter plate, as the largest proportion of mesenchymal stem cells adhered to this material. We did not get consistent results on the other materials, therefore we did not come to clear conclusions. We have confirmed that cell-material interactions are very complex and still need to be researched in detail. Finding new methods for cell separation is difficult, as it is impossible to determine simple criteria for successful adhesion and following cell separation.
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