Human gut microbiota plays an important role in human life. Intestinal microbiota is involved in food metabolism, producing short-chain fatty acids and vitamins, protects the host against infection by pathogens and affect the development of the host's immune system. Spore-forming bacteria in human intestinal microbiota because of easy spore transfer allow establishment of homeostasis after dysbiosis and have a potential for use as probiotics. To know the interactions between the intestinal microbiota and the host and to allow research on intestinal bacteria it is necessary to obtain a pure culture, which can only grow on solid media. The aim of our master thesis was to optimize cultivation of sporogenic fraction from human fecal samples. Four faecal samples were treated with alcohol shock. Fistly we incoculated sporogenic fractions on solid media with the addition of fecal suspension or rumen fluid. Than we also examined the impact of enrichment of the sporogenic fractions on growth of spore-forming bacteria on solid media. Four different enrichment media were used with different available carbon sources. Use of feacal suspension compared to other approaches increased the number of different bacterial groups on solid media. Enrichment allowed the growth of some new bacterial groups but also prevented the reproduction of some others. The inoculation of enrichment on solid media compared to the direct inoculation of the sporogenic fraction did not increase the number of different bacterial groups, but it did allow the detection of new unidentified bacterial groups. Based on the obtained results we can conclude that the isolation of a wide range of different spore-forming bacterial species from human feces requires the use of several different media.
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