Supercritical carbon dioxide extraction of four medicinal mediterranean plants : investigation of chemical composition and antioxidant activity

Čižmek, Lara (avtor), Bavcon Kralj, Mojca (avtor), Čož-Rakovac, Rozelindra (avtor), Mazur, Dmitry M. (avtor), Ul'yanovskii, Nikolai (avtor), Likon, Marko (avtor), Trebše, Polonca (avtor)

With everyday advances in the field of pharmaceuticals, medicinal plants have high priority regarding the introduction of novel synthetic compounds by the usage of environmentally friendly extraction technologies. Herein, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The extraction yield was the highest for the chamomile (5%), while moderate yields were obtained for the other three plants. The chemical composition analyzed by gas chromatographyhigh-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1,1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2 = 0.818) and ferric reducing antioxidant power (FRAP) assay (R2 = 0.640), but not to the total phenolic content (R 2 = 0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest.

• Jezik: Angleški jezik
• Ključne besede: green solvents, chamomile, yarrow, St. John's wort, curry plant, square-wave voltammetry, chromatography
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: ZF - Zdravstvena fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: Str. 1-18
• Številčenje: Vol. 26, iss. 18
• PID: 20.500.12556/RUL-131188
• UDK: 543.544
• ISSN pri članku: 1420-3049
• DOI: 10.3390/ molecules26185697
• COBISS.SI-ID: 77584387
• Datum objave v RUL: 23.09.2021

Gradivo je del revije

• Naslov: Molecules
• Skrajšan naslov: Molecules
• Založnik: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 20.09.2021

Sekundarni jezik

• Jezik: Ni določen
• Ključne besede: zelena topila, kamilica, rman, šentjanževka, curry, voltametrija s kvadratnimi valovi, kromatografija

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J2-8162
• Naslov: Zapiranje snovnih poti pri čiščenju komunalnih odpadnih voda z zelenimi tehnologijami

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0388
• Naslov: Mehanizmi varovanja zdravja