Supercritical carbon dioxide extraction of four medicinal mediterranean plants : investigation of chemical composition and antioxidant activity

Čižmek, Lara (author), Bavcon Kralj, Mojca (author), Čož-Rakovac, Rozelindra (author), Mazur, Dmitry M. (author), Ul'yanovskii, Nikolai (author), Likon, Marko (author), Trebše, Polonca (author)

With everyday advances in the field of pharmaceuticals, medicinal plants have high priority regarding the introduction of novel synthetic compounds by the usage of environmentally friendly extraction technologies. Herein, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The extraction yield was the highest for the chamomile (5%), while moderate yields were obtained for the other three plants. The chemical composition analyzed by gas chromatographyhigh-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1,1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2 = 0.818) and ferric reducing antioxidant power (FRAP) assay (R2 = 0.640), but not to the total phenolic content (R 2 = 0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest.

• Language: English
• Keywords: green solvents, chamomile, yarrow, St. John's wort, curry plant, square-wave voltammetry, chromatography
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: ZF - Faculty of Health Sciences
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: Str. 1-18
• Numbering: Vol. 26, iss. 18
• PID: 20.500.12556/RUL-131188
• UDC: 543.544
• ISSN on article: 1420-3049
• DOI: 10.3390/ molecules26185697
• COBISS.SI-ID: 77584387
• Publication date in RUL: 23.09.2021

Record is a part of a journal

• Title: Molecules
• Shortened title: Molecules
• Publisher: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 20.09.2021

Secondary language

• Language: Undetermined
• Keywords: zelena topila, kamilica, rman, šentjanževka, curry, voltametrija s kvadratnimi valovi, kromatografija

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: J2-8162
• Name: Zapiranje snovnih poti pri čiščenju komunalnih odpadnih voda z zelenimi tehnologijami

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0388
• Name: Mehanizmi varovanja zdravja