In vitro innervation as an experimental model to study the expression and functions of acetylcholinesterase and agrin in human skeletal muscle

Miš, Katarina (avtor), Grubič, Zoran (avtor), Lorenzon, Paola (avtor), Sciancalepore, Marina (avtor), Marš, Tomaž (avtor), Pirkmajer, Sergej (avtor)

Acetylcholinesterase (AChE) and agrin, a heparan-sulfate proteoglycan, reside in the basal lamina of the neuromuscular junction (NMJ) and play key roles in cholinergic transmission and synaptogenesis. Unlike most NMJ components, AChE and agrin are expressed in skeletal muscle and α-motor neurons. AChE and agrin are also expressed in various other types of cells, where they have important alternative functions that are not related to their classical roles in NMJ. In this review, we first focus on co-cultures of embryonic rat spinal cord explants with human skeletal muscle cells as an experimental model to study functional innervation in vitro. We describe how this heterologous rat-human model, which enables experimentation on highly developed contracting human myotubes, offers unique opportunities for AChE and agrin research. We then highlight innovative approaches that were used to address salient questions regarding expression and alternative functions of AChE and agrin in developing human skeletal muscle. Results obtained in co-cultures are compared with those obtained in other models in the context of general advances in the field of AChE and agrin neurobiology.

• Jezik: Angleški jezik
• Ključne besede: acetylcholinesterase, human muscle, apoptosis, in vitro innervation, neuromuscular junction, co-cultures, agrin
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.02 - Pregledni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2017
• Št. strani: 30 str.
• Številčenje: Vol. 22, iss. 9, art. 1418
• PID: 20.500.12556/RUL-131011
• UDK: 616-092
• ISSN pri članku: 1420-3049
• DOI: 10.3390/molecules22091418
• COBISS.SI-ID: 33379545
• Datum objave v RUL: 21.09.2021

Gradivo je del revije

• Naslov: Molecules
• Skrajšan naslov: Molecules
• Založnik: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.09.2017

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: acetilholinesteraza, človeška mišica, apoptoza