Antioxidative effects of olive polyphenols compared to vitamin E in piglets fed a diet rich in N-3 PUFA

Leskovec, Jakob (author), Rezar, Vida (author), Nemec Svete, Alenka (author), Salobir, Janez (author), Levart, Alenka (author)

The aim of the current study was to assess the antioxidative effects of the dietary supplementation of olive leaf extract (OLE) in different concentrations compared to those of vitamin E in piglets under conditions of dietary n-3 PUFA-induced oxidative stress. Forty-eight castrated male piglets (10.6 ± 0.99 kg) were fed the following experimental diets: Cont− (low-fat diet, no supplement), Cont+ (high linseed oil diet, no supplement), Vit-E (as Cont+, 105 IU vitamin E/day), OLE-1 (as Cont+, 3.84 mg hydroxytyrosol equivalents (HEQs)/day), OLE-2 (as Cont+, 38.4 mg HEQ/day), and OLE-3 (as Cont+, 96 mg HEQ/day). After 21 days of feeding, the experimental diets, blood and urine samples were collected to assess the extent of the oxidative stress. Results indicated that diet OLE-1 lowered the activity of gamma-glutamyl transferase, protected DNA (measured as DNA tail %) and altered urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG). Dietary vitamin E lowered the levels of urinary F$_2$-isoprostanes, as well as of plasma malondialdehyde and γ-tocopherol, but raised the plasmatic α-tocopherol and altered the level of urinary 8-OHdG. In conclusion, only minor positive effects of dietary OLE on the oxidative stress parameters were observed. Additionally, OLE did not show concentration dependence.

• Language: English
• Keywords: hydroxytyrosol, linseed oil, olive polyphenols, oxidative stress, piglets, vitamin E
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty; VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2019
• Number of pages: 11 str.
• Numbering: Vol. 9, iss. 4, art. 161
• PID: 20.500.12556/RUL-130315
• UDC: 636.4.084/.087
• ISSN on article: 2076-2615
• DOI: 10.3390/ani9040161
• COBISS.SI-ID: 4204936
• Publication date in RUL: 13.09.2021

Record is a part of a journal

• Title: Animals
• Shortened title: Animals
• Publisher: MDPI AG
• ISSN: 2076-2615
• COBISS.SI-ID: 519120409

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 12.04.2019

Secondary language

• Language: Slovenian
• Keywords: prašiči, pujski, prehrana živali, maščobne kisline, PUFA, olive, polifenoli, vitamini, vitamin E

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0097
• Name: Prehrana in mikrobna ekologija prebavil