Nicotinamide mononucleotide (NMN) is a molecule that, due to its pharmacological effects, has recently gained a large interest in the pharmaceutical field. As it has a beneficial effect on aging and related diseases, many manufacturers strive to place such a dietary supplement on the market themselves. However, if they want to produce NMN, it is necessary to be able to determine and measure its content, which is the topic of my thesis.
I wanted to explore the possibilities of determining this type of compound using the HPLC technique, focusing on finding the optimal conditions for chromatographic analysis. I tested different chromatographic columns, mobile phases with different pH values, different column temperatures and gradients. Based on the obtained results, I determined the optimal separation conditions and characterized the method with the parameters of linearity and repeatability.
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