Active protein aggregates produced in Escherichia coli

Peternel, Špela (author), Komel, Radovan (author)

Since recombinant proteins are widely used in industry and in research, the need for their low-cost production is increasing. Escherichia coli is one of the best known and most often used host organisms for economical protein production. However, upon over-expression, protein aggregates called inclusion bodies (IBs) are often formed. Until recently IBs formation represented a bottleneck in protein production as they were considered as deposits of inactive proteins. However, recent studies show that by choosing the appropriate host strain and designing an optimal production process, IBs composed from properly folded and biologically active recombinant proteins can be prepared. Such active protein particles can be further used for the isolation of pure proteins or as whole active protein particles in various biomedical and other applications. Therefore interest in understanding the mechanisms of their formation as well as their properties is increasing.

• Language: English
• Keywords: E. coli, active protein aggregates, inclusion bodies, IBs, recombinant proteins, biotechnology
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2011
• Number of pages: Str. 8275-8287
• Numbering: Vol. 12, iss. 11
• PID: 20.500.12556/RUL-129622
• UDC: 577
• ISSN on article: 1422-0067
• DOI: 10.3390/ijms12118275
• COBISS.SI-ID: 29115609
• Publication date in RUL: 06.09.2021

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licences

• License: CC BY 3.0, Creative Commons Attribution 3.0 Unported
• Link: https://creativecommons.org/licenses/by/3.0/deed.en
• Description: You are free to reproduce and redistribute the material in any medium or format. You are free to remix, transform, and build upon the material for any purpose, even commercially. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
• Licensing start date: 22.11.2011

Secondary language

• Language: Slovenian
• Keywords: Escherichia coli, rekombinantne beljakovine, inkluzijska telesca, biotehnologija