Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support

Babič, Janja (avtor), Likozar, Blaž (avtor), Pavko, Aleksander (avtor)

Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L$^{−1}$ and 16 U L$^{−1}$ day$^{−1}$ for laccase (Lac), and 193 U L$^{−1}$ and 80 U L$^{−1}$ day$^{−1}$ for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L$^{−1}$ for Lac and 58 U L$^{−1}$ for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

• Jezik: Angleški jezik
• Ključne besede: laccase, manganese peroxidase, ligninolytic enzymes, enzyme production, medium optimization, submerged cultivation, immobilized culture, bioremediation, responce surface methodology, decolorization
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2012
• Št. strani: Str. 11365-11384
• Številčenje: Vol. 13, iss. 9
• PID: 20.500.12556/RUL-129511
• UDK: 66.098:602.4:577.15:579
• ISSN pri članku: 1661-6596
• DOI: 10.3390/ijms130911365
• COBISS.SI-ID: 36176901
• Datum objave v RUL: 03.09.2021

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1661-6596
• COBISS.SI-ID: 36217605

Licence

• Licenca: CC BY 3.0, Creative Commons Priznanje avtorstva 3.0 Nedoločena
• Povezava: https://creativecommons.org/licenses/by/3.0/deed.sl
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• Začetek licenciranja: 12.09.2012

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: biološko inženirstvo, biotehnologija, mikrobiologija, lignin, razbarvanje, ligninolitični encimi, lakaze, mangan peroksidaza, produkcija encimov, optimizacija, bioreaktorji, glive, gojenje gliv, gliva Ceriporiopsis subvermispora

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P2-0152
• Naslov: Kemijsko reakcijsko inženirstvo