Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support

Babič, Janja (author), Likozar, Blaž (author), Pavko, Aleksander (author)

Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L$^{−1}$ and 16 U L$^{−1}$ day$^{−1}$ for laccase (Lac), and 193 U L$^{−1}$ and 80 U L$^{−1}$ day$^{−1}$ for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L$^{−1}$ for Lac and 58 U L$^{−1}$ for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

• Language: English
• Keywords: laccase, manganese peroxidase, ligninolytic enzymes, enzyme production, medium optimization, submerged cultivation, immobilized culture, bioremediation, responce surface methodology, decolorization
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Year: 2012
• Number of pages: Str. 11365-11384
• Numbering: Vol. 13, iss. 9
• PID: 20.500.12556/RUL-129511
• UDC: 66.098:602.4:577.15:579
• ISSN on article: 1661-6596
• DOI: 10.3390/ijms130911365
• COBISS.SI-ID: 36176901
• Publication date in RUL: 03.09.2021

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1661-6596
• COBISS.SI-ID: 36217605

Licences

• License: CC BY 3.0, Creative Commons Attribution 3.0 Unported
• Link: https://creativecommons.org/licenses/by/3.0/deed.en
• Description: You are free to reproduce and redistribute the material in any medium or format. You are free to remix, transform, and build upon the material for any purpose, even commercially. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
• Licensing start date: 12.09.2012

Secondary language

• Language: Slovenian
• Keywords: biološko inženirstvo, biotehnologija, mikrobiologija, lignin, razbarvanje, ligninolitični encimi, lakaze, mangan peroksidaza, produkcija encimov, optimizacija, bioreaktorji, glive, gojenje gliv, gliva Ceriporiopsis subvermispora

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P2-0152
• Name: Kemijsko reakcijsko inženirstvo