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The comparison of honeybee viral loads for six honeybee viruses (ABPV, BQCV, CBPV, DWV, LSV3 and SBV) in healthy and clinically affected honeybees with TaqMan quantitative real-time RT-PCR assays
ID
Šimenc, Laura
(
Avtor
),
ID
Knific, Tanja
(
Avtor
),
ID
Toplak, Ivan
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(2,05 MB)
MD5: 35747205901F48F2540E359A306315D6
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/1999-4915/13/7/1340
Galerija slik
Izvleček
The viral loads of acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), Lake Sinai virus 3 (LSV3), and sacbrood bee virus (SBV) were determined in samples with the use of quantitative TaqMan real-time reverse transcription and polymerase chain reaction (RT-qPCR). A total of 108 samples of healthy adult honeybees from four differently located apiaries and samples of honeybees showing different clinical signs of viral infections from 89 apiaries were collected throughout Slovenia. The aim of this study was to discover correlations between viral loads and clinical signs in adult honeybees and confirm previously set threshold viral load levels between healthy and clinically affected honeybees. Within this study, two new RT-qPCR assays for quantification of LSV3 and SBV were developed. Statistically significant differences in viral loads of positive samples were identified between healthy and clinically affected honeybees for ABPV, CBPV, DWV, and SBV, while for BQCV and LSV3, no statistical differences were observed between both groups. Despite high detected LSV3 prevalence and viral loads around 6.00 log$_{10}$ viral copies/bee, this lineage probably has a limited impact on the health status of honeybee colonies. The determined viral loads between 3.94 log$_{10}$ and 13.17 log$_{10}$ in positive samples for six viruses, collected over 10 consecutive months, including winter, present additional information of high viral load variations in healthy honeybee colonies.
Jezik:
Angleški jezik
Ključne besede:
honeybees
,
viral load
,
monthly sampling
,
RT-qPCR
,
prevalence
,
bees
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
VF - Veterinarska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2021
Št. strani:
17 str.
Številčenje:
Vol. 13, iss. 7, art. 1340
PID:
20.500.12556/RUL-128772
UDK:
638:616:578
ISSN pri članku:
1999-4915
DOI:
10.3390/v13071340
COBISS.SI-ID:
70326275
Datum objave v RUL:
28.07.2021
Število ogledov:
817
Število prenosov:
148
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Viruses
Skrajšan naslov:
Viruses
Založnik:
MDPI
ISSN:
1999-4915
COBISS.SI-ID:
517597977
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
11.07.2021
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Program financ.:
Young researchers
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P4-0092
Naslov:
Zdravje živali, okolje in varna hrana
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