The comparison of honeybee viral loads for six honeybee viruses (ABPV, BQCV, CBPV, DWV, LSV3 and SBV) in healthy and clinically affected honeybees with TaqMan quantitative real-time RT-PCR assays

Šimenc Kramar, Laura (avtor), Knific, Tanja (avtor), Toplak, Ivan (avtor)

The viral loads of acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), Lake Sinai virus 3 (LSV3), and sacbrood bee virus (SBV) were determined in samples with the use of quantitative TaqMan real-time reverse transcription and polymerase chain reaction (RT-qPCR). A total of 108 samples of healthy adult honeybees from four differently located apiaries and samples of honeybees showing different clinical signs of viral infections from 89 apiaries were collected throughout Slovenia. The aim of this study was to discover correlations between viral loads and clinical signs in adult honeybees and confirm previously set threshold viral load levels between healthy and clinically affected honeybees. Within this study, two new RT-qPCR assays for quantification of LSV3 and SBV were developed. Statistically significant differences in viral loads of positive samples were identified between healthy and clinically affected honeybees for ABPV, CBPV, DWV, and SBV, while for BQCV and LSV3, no statistical differences were observed between both groups. Despite high detected LSV3 prevalence and viral loads around 6.00 log$_{10}$ viral copies/bee, this lineage probably has a limited impact on the health status of honeybee colonies. The determined viral loads between 3.94 log$_{10}$ and 13.17 log$_{10}$ in positive samples for six viruses, collected over 10 consecutive months, including winter, present additional information of high viral load variations in healthy honeybee colonies.

• Jezik: Angleški jezik
• Ključne besede: honeybees, viral load, monthly sampling, RT-qPCR, prevalence, bees
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 17 str.
• Številčenje: Vol. 13, iss. 7, art. 1340
• PID: 20.500.12556/RUL-128772
• UDK: 638:616:578
• ISSN pri članku: 1999-4915
• DOI: 10.3390/v13071340
• COBISS.SI-ID: 70326275
• Datum objave v RUL: 28.07.2021

Gradivo je del revije

• Naslov: Viruses
• Skrajšan naslov: Viruses
• Založnik: MDPI
• ISSN: 1999-4915
• COBISS.SI-ID: 517597977

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 11.07.2021

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Program financ.: Young researchers

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0092
• Naslov: Zdravje živali, okolje in varna hrana