Accumulation of agmatine, spermidine, and spermine in sprouts and microgreens of alfalfa, fenugreek, lentil, and daikon radish

Kralj Cigić, Irena (author), Rupnik, Sašo (author), Rijavec, Tjaša (author), Poklar Ulrih, Nataša (author), Cigić, Blaž (author)

Sprouts and microgreens are a rich source of various bioactive compounds. Seeds of lentil, fenugreek, alfalfa, and daikon radish seeds were germinated and the contents of the polyamines agmatine (AGM), putrescine (PUT), cadaverine (CAD), spermidine (SPD), and spermine (SPM) in ungerminated seeds, sprouts, and microgreens were determined. In general, sprouting led to the accumulation of the total polyamine content. The highest levels of AGM (5392 mg/kg) were found in alfalfa microgreens, PUT (1079 mg/kg) and CAD (3563 mg/kg) in fenugreek sprouts, SPD (579 mg/kg) in lentil microgreens, and SPM (922 mg/kg) in fenugreek microgreens. A large increase in CAD content was observed in all three legume sprouts. Conversely, the nutritionally beneficial polyamines AGM, SPD, and SPM were accumulated in microgreens, while their contents of CAD were significantly lower. In contrast, daikon radish sprouts exhibited a nutritionally better profile of polyamines than the microgreens. Freezing and thawing of legume sprouts resulted in significant degradation of CAD, PUT, and AGM by endogenous diamine oxidases. The enzymatic potential of fenugreek sprouts can be used to degrade exogenous PUT, CAD, and tyramine at pH values above 5.

• Language: English
• Keywords: polyamines, biogenic amines, germination, medicago sativa, trigonella foenum-graecum, lens culinaris, raphanus sativus, diamine oxidase
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology; BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 20 str.
• Numbering: Vol. 9, iss. 5, art. 547
• PID: 20.500.12556/RUL-128311
• UDC: 577.1:547.233:641.1:543.544
• ISSN on article: 2304-8158
• DOI: 10.3390/foods9050547
• COBISS.SI-ID: 13634563
• Publication date in RUL: 08.07.2021

Record is a part of a journal

• Title: Foods
• Shortened title: Foods
• Publisher: MDPI
• ISSN: 2304-8158
• COBISS.SI-ID: 512252472

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.05.2020

Secondary language

• Language: Slovenian
• Keywords: biogeni amini, poliamini, agmatin, spermidin, spermin, kalčki, kalitev, ekstrakcija, sušenje, zmrzovanje, tajanje, kromatografske metode, HPLC

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0121
• Name: Biokemijska in biofizikalno-kemijska karakterizacija naravnih snovi

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0153
• Name: Raziskave in razvoj analiznih metod in postopkov