Peritonitis and peritoneal wall thickening are common complications of peritoneal dialysis, which affect further dialysis and can even end with death. Histology of peritoneal membrane and PCR analysis are currently used for condition assessment. Histology is very accurate, but can only process small number of samples with limited size. PCR analysis can only detect changes on bigger areas without spatial resolution. Because these methods have their limitations, true condition can be easily undetected so development of new technique, which can give us more data and can examine bigger area, is urgently needed.
Mesoscopic hyperspectral imaging with wavelengths between 400 and 1000 nm was used to assess changes on abdominal wall and effects of medications Resolvin and Epiresolvin on inflammation process by measuring spectrum of transmitted light. Study involved 17 murine males divided in several groups with induced inflammation with Chlorhexidine, treated with Resolvin and Epiresolvin and healthy controls.
Scattering and absorption characteristics were determined by fitting model of light - tissue interactions onto hyperspectral data. Spatial distributions of chromophores, scattering coefficients and scattering power were obtained. Statistical metrics were determined with statistical tools, which could represent abdominal wall inflammation biomarkers.
Results of hyperspectral imaging were compared to those of PCR and histology analysis. All of the methods had similar findings and good agreement.
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