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Preučevanje elektroporacije na gomolju krompirja : magistrsko delo
ID Kregar, Martin (Author), ID Kranjc, Matej (Mentor) More about this mentor... This link opens in a new window

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Abstract
Elektroporacija je pojav, pri katerem pride do povečanja prepustnosti celične membrane zaradi izpostavitve celice električnemu polju. Elektroporacija je bila in je še vedno predmet intenzivnih raziskav na številnih področjih, kot so biomedicina, v predelavi hrane in kemijskem inženirstvu, prav tako pa tudi v okolijskih znanostih. Pri veliko študijah se uporablja za preučevanje porazdelitve električnega polja pri elektroporaciji surov gomolj krompirja, saj območje, ki je izpostavljeno elektroporaciji, zaradi oksidacije fenolnih spojin potemni že v roku 5 ur po dovajanju elektroporacijskih pulzov. Cilj magistrskega dela je preučevanje elektroporacije na gomolju krompirja in določitev pragovne vrednosti električnega polja za dosego ireverzibilne elektroporacije. Za ta namen sem izvedel poskuse na različnih sortah gomolja krompirja pri različnih amplitudah dovedenih pulzov, meritev impedance pred in po elektroporaciji in meritev napetosti ter toka dovedenih električnih pulzov. Analiza elektroporiranega dela gomolja krompirja je temeljila na zajemu slik gomolja in segmentaciji po izvedeni elektroporaciji. Za ovrednotenje in določevanje praga ireverzibilne elektroporacije sem izdelal numerični model gomolja krompirja in s pomočjo metode končnih elementov izračunal porazdelitev električnega polja, ki se vzpostavi med elektroporacijo. S primerjavo površine numeričnega modela in segmentiranega dela gomolja sem določil pragovno vrednost električnega polja, za doseganje ireverzibilne elektroporacije na gomolju krompirja.

Language:Slovenian
Keywords:elektroporacija, gomolj krompirja, metoda končnih elementov, električno polje, segmentacija
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FE - Faculty of Electrical Engineering
Place of publishing:Ljubljana
Publisher:[M. Kregar]
Year:2021
Number of pages:67 str.
PID:20.500.12556/RUL-127949 This link opens in a new window
UDC:602.621:582.930.11(043.2)
COBISS.SI-ID:68691715 This link opens in a new window
Publication date in RUL:30.06.2021
Views:1699
Downloads:229
Metadata:XML DC-XML DC-RDF
:
KREGAR, Martin, 2021, Preučevanje elektroporacije na gomolju krompirja : magistrsko delo [online]. Master’s thesis. Ljubljana : M. Kregar. [Accessed 2 April 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=127949
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Secondary language

Language:English
Title:Study of electroporation on potato tuber
Abstract:
Electroporation is a phenomenon where is an increase in permeability of the cell membrane due to the exposure of the cell to an electric field. Electroporation has been and continues to be the subject of intensive research in many fields, such as biomedicine, food processing and chemical engineering, as well as in environmental sciences. In many studies, a raw potato tuber is used to study electric field distribution established during electroporation, as exposed area darkens due to oxidation of phenolic compounds within 5 hours after electroporation pulses. The aim of thesis is to study electroporation on potato tuber and determine the threshold of electric field to achieve irreversible electroporation. For this purpose, we performed experiments on different varieties of potato tubers at different delivered pulses amplitudes, measuring the impedance before and after electroporation and measuring the voltage and current of the supplied electric pulses. The analysis of the electroporated part of the potato tuber was based on the captured images of the potato tuber and segmentation after electroporation. We made a numerical model of the potato tuber using finite element method to evaluate and determine threshold of irreversible electroporation and calculated distribution of the electric field during electroporation. The threshold value of the electric field for irreversible electroporation was determined by comparing area of the numerical model and segmented part of the potato tuber.

Keywords:electroporation, potato tuber, finite element method, electric field, segmentation

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