Application of confocal laser scanning microscopy in dendrochronology

Balzano, Angela (author), Novak, Klemen (author), Humar, Miha (author), Čufar, Katarina (author)

We used the Confocal Laser Scanning Microscope (CLSM) Olympus LEXT OLS5000 for non-destructive observation and image analysis of wood anatomy traits in growth layers of tree species from different climatic zones. In European beech (Fagus sylvatica), where tree rings can generally be recognised, we discuss the changes in tree-ring structure due to adverse effects (insect attacks). Growth layers in Mediterranean Aleppo pine (Pinus halepensis) from south-eastern Spain are not always annual and contain numerous intra-annual density fluctuations (IADFs). Ocote pine (Pinus oocarpa) growing at high elevation in Honduras showed growth layers with clear growth ring boundaries and IADFs. In both pines, CLSM allowed us to recognise and measure tracheid parameters to define density fluctuations. In tropical true mahogany (Swietenia macrophylla) from Venezuela and cedrela (Cedrela odorata) from Costa Rica, we studied the growth layers with variable dimensions of vessels demarcated by marginal axial parenchyma.

• Language: English
• Keywords: confocal laser scanning microscopy, wood anatomy, wood identification, dendrochronology, image analysis, tree-rings
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2019
• Number of pages: Str. 5-17
• Numbering: Letn. 68, št. 2
• PID: 20.500.12556/RUL-126631
• UDC: 630*561.24:537.533.35
• ISSN on article: 0024-1067
• DOI: 10.26614/les-wood.2019.v68n02a01
• COBISS.SI-ID: 3167881
• Publication date in RUL: 29.04.2021

Record is a part of a journal

• Title: Les
• Shortened title: Les
• Publisher: Zveza društev inženirjev in tehnikov gozdarstva in lesarstva Slovenije, Zveza društev inženirjev in tehnikov gozdarstva in lesarstva Slovenije, Biotehniška fakulteta, Oddelek za lesarstvo, Založba Univerze, Biotehniška fakulteta, Oddelek za lesarstvo, Založba Univerze
• ISSN: 0024-1067
• COBISS.SI-ID: 13940224

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 29.04.2021

Secondary language

• Language: Slovenian
• Title: Uporaba konfokalne laserske vrstične mikroskopije v dendrokronologiji

Abstract

Konfokalni laserski vrstični mikroskop (CLSM) Olympus LEXT OLS5000 smo uporabili za ne destruktivno proučevanje anatomskih znakov v prirastnih plasteh dreves iz različnih podnebnih pasov. Pri bukvi (Fagus sylvatica), kjer je na splošno mogoče prepoznati branike, CLSM omogoča študij anatomskih posebnosti zaradi škodljivih učinkov (npr. napadov žuželk). Prirastne plasti sredozemskega alepskega bora (Pinus halepensis) v jugovzhodni Španiji ne nastajajo vedno v skladu s koledarskim letom, zaznamujejo jih namreč tudi številna nihanja gostote (IADFs) znotraj posameznih plasti. Bor Pinus oocarpa, ki raste na visoki nadmorski višini v Hondurasu, izkazuje jasno razmejene prirastne plasti in pojav IADFs. V obeh borih smo s pomočjo CLSM prepoznali in izmerili dimenzije traheid za boljše določanje IADF in za boljšo razmejitev prirastnih plasti. V lesu srednjeameriškega mahagonija (Swietenia macrophylla) iz Venezuele in cedrele (Cedrela odorata) iz Kostarike smo analizirali prirastne plasti, ki imajo spremenljive dimenzije trahej in so razmejene z marginalnim aksialnim parenhimom.

• Keywords: konfokalna laserska vrstična mikroskopija, anatomija lesa, analiza slike, branike, dendrokronologija, prirastne plasti

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0015
• Name: Les in lignocelulozni kompoziti

• Funder: Other - Other funder or multiple funders
• Funding programme: Researchers 2.1-UL-BF-952011
• Project number: CGL 2015-69985_R (CLIMED)