Efficient inactivation of MS-2 virus in water by hydrodynamic cavitation

Kosel, Janez (author), Gutiérrez-Aguirre, Ion (author), Rački, Nejc (author), Dreo, Tanja (author), Ravnikar, Maja (author), Dular, Matevž (author)

The aim of this study was to accurately quantify the impact of hydrodynamic cavitation on the infectivity of bacteriophage MS2, a norovirus surrogate, and to develop a small scale reactor for testing the effect of hydrodynamic cavitation on human enteric viruses, which cannot be easily prepared in large quantities. For this purpose, 3 mL scale and 1 L scale reactors were constructed and tested. Both devices were efficient in generating hydrodynamic cavitation and in reducing the infectivity of MS2 virus. Furthermore, they reached more than 4 logs reductions of viral infectivity, thus confirming the scalability of hydrodynamic cavitation for this particular application. As for the mechanism of page inactivation, we suspect that cavitation generated OH$^-$ radicals formed an advanced oxidation process, which could have damaged the host's recognition receptors located on the surface of the bacteriophage. Additional damage could arise from the high shear forces inside the cavity. Moreover, the effectiveness of the cavitation was higher for suspensions containing low initial viral titers that are in similar concentration to the ones found in real water samples. According to this, cavitation generators could prove to be a useful tool for treating virus-contaminated wastewaters in the future.

• Language: English
• Keywords: MS2 bacteriophage, norovirus surrogate, hydrodynamic cavitation, Venturi type constriction, phage infectivity
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FS - Faculty of Mechanical Engineering
• Publication status: Published
• Publication version: Author Accepted Manuscript
• Year: 2017
• Number of pages: Str. 465-471
• Numbering: Vol. 124
• PID: 20.500.12556/RUL-126446
• UDC: 578.2:532(045)
• ISSN on article: 0043-1354
• DOI: 10.1016/j.watres.2017.07.077
• COBISS.SI-ID: 15600923

Copyright

Zapis Sherpa Romeo za revijo Water research https://v2.sherpa.ac.uk/id/publication/13286 : licenca za recenzirani rokopis (Accepted Version) je CC BY-NC-ND 4.0. (Datum opombe: 15. 3. 2023)

• Publication date in RUL: 22.04.2021

Record is a part of a journal

• Title: Water research
• Shortened title: Water res.
• Publisher: Elsevier
• ISSN: 0043-1354
• COBISS.SI-ID: 26617344

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
• Licensing start date: 01.11.2017

Secondary language

• Language: Slovenian
• Keywords: kavitacija, MS2 bakteriofag, venturijeva cev, virusi, infektivnost