Keratin dynamics and spatial distribution in wild-type and K14 R125P mutant cells—a computational model

Gouveia, Marcos (avtor), Zemljič Jokhadar, Špela (avtor), Vidak, Marko (avtor), Stojković, Biljana (avtor), Derganc, Jure (avtor), Travasso, Rui (avtor), Liović, Mirjana (avtor)

Keratins are one of the most abundant proteins in epithelial cells. They form a cytoskeletal filament network whose structural organization seriously conditions its function. Dynamic keratin particles and aggregates are often observed at the periphery of mutant keratinocytes related to the hereditary skin disorder epidermolysis bullosa simplex, which is due to mutations in keratins 5 and 14. To account for their emergence in mutant cells, we extended an existing mathematical model of keratin turnover in wild-type cells and developed a novel 2D phase-field model to predict the keratin distribution inside the cell. This model includes the turnover between soluble, particulate and filamentous keratin forms. We assumed that the mutation causes a slowdown in the assembly of an intermediate keratin phase into filaments, and demonstrated that this change is enough to account for the loss of keratin filaments in the cell's interior and the emergence of keratin particles at its periphery. The developed mathematical model is also particularly tailored to model the spatial distribution of keratins as the cell changes its shape.

• Jezik: Angleški jezik
• Ključne besede: keratin, epidermolysis bullosa simplex, mutation, phase-field model, reaction-diffusion-advection equation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 14 str.
• Številčenje: Vol. 21, iss. 7, art. 2596
• PID: 20.500.12556/RUL-125685
• UDK: 61
• ISSN pri članku: 1422-0067
• DOI: 10.3390/ijms21072596
• COBISS.SI-ID: 34772697
• Datum objave v RUL: 01.04.2021

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 09.04.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: keratin, epidermoliza bullosa simplex, mutacija, model faznega polja, enačba reakcije-difuzije-advekcije