The biomedical utility of genetically modified immune cells (especially lymphocytes T) shows great value in treatment malignant diseases. Lymphocytes T can express various molecular constructs for therapeutic substances, that can influence the course of the disease. For the introduction of mRNA of molecular constructs of chimeric antigen receptors (CAR T), that we wanted to insert into the cells of cell lines Jurkat in the task, we used electrogenic transfer. By optimizing the procedures, we defined the useful conditions for inserting these into human lymphocites T. Lymphocytes T would be taken from the patient, changed in the laboratory and expanded, then returned to the patient's peripheral blood, where the protein of construct would be expressed and perform its task. The mRNA of the molecular construct α-CAR-CD19 was expressed in 22 and 40,2 % of cells of cell line Jurkat at 1000 V or 1100 V, a pulse length of 40 ms. With proper pre-preparation and successfully transcribed molecular construct into mRNA in the process of transcription in vitro, we can be very successful in electro gene transfer of vectors into cells under given conditions. The most important procedure in the preparation is the full modification of the mRNA.
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