In 2019, we set up an experiment with four different cultivars with four replicates in a greenhouse of the Biotechnical Faculty Department of Agronomy in Ljubljana. We wanted to check how the cultivars ('Kozjak', 'Avbar') of the European chestnut are propagated in comparison with the cultivars that are hybrids between the European chestnut and the Japanese chestnut ('Marsol’ and' ‘Maraval '). We used 12 cm long top cuttings. The cuttings were first treated with the hormone auxin. Then they were put them into a 1: 1 mixture of sand and peat, to which we added the slowly soluble Osmocote fertilizer. After the end of the growing season, the cuttings were evaluated. We evaluated several different parameters: rooting, shoot length, survival, measured callus. The cultivar 'Maraval' had the best rooting (22.4 %), while the varieties 'Avbar' and 'Kozjak' had the worst rooting (6.6 %). The most callus was developed by the cultivar 'Avbar' (67.2 %), and the least by the cultivar 'Marsol' (15.7 %). Varieties 'Avbar' and 'Kozjak' rooted only basally, while cultivars 'Marsol' and 'Maraval' also rooted acrobasally. It was also the largest callus and root development in the ‘Avbar’ cultivar. The average number of roots was highest in the cultivars 'Maraval' and 'Marsol' (2.1-2.3). The longest root bunch was measured in the cultivar 'Kozjak' (20.5 cm). In the cultivar 'Maraval', we measured an average shoot growth of 11.2 cm. The average number of shoots was highest in the cultivars 'Maraval' and 'Kozjak' (2.5 and 2.3).
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