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Primerjava protimikrobnega učinka kovinskih nanodelcev in njihovega topnega ionskega dela na bakterije Staphylococcus aureus in Escherichia coli
ID Semič, Melita (Author), ID Marinšek Logar, Romana (Mentor) More about this mentor... This link opens in a new window, ID Modic, Martina (Comentor)

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Abstract
Pri praktičnemu delu magistrske naloge smo uporabljali komercialno dostopne Ag100, Ag20-30, ZnO, Cu in SiO2 nanodelce. Preučevali smo protibakterijske učinke kovinskih nanodelcev in sproščenih ionov. Obliko nanodelcev smo karakterizirali s transmisijsko elektronsko mikroskopijo (TEM). Vpliv ionov in nanodelcev na bakterije Escherichia coli in Staphylococcus aureus smo določili s spektroskopskimi meritvami optičnih gostot (OD600) in metodo štetja kolonij na trdem gojišču (CFU), vpliv ionov na humane celice smo določili s testi citotoksičnosti in točno koncentracijo sproščenih ionov z masno spektrometrijo z induktivno sklopljeno plazmo (ICP-MS). Vpliv kovinskih nanodelcev na morfologijo bakterij v biofilmih smo preverjali z vrstično elektronsko mikroskopijo (SEM). Pri E. coli je minimalna inhibitorna koncentracija (MIK) Ag20-30 ionov med 0,64 in 0,85 µg/mL. Pri S. aureus znašajo MIK Ag20-30, ZnO in Cu ionov 0,85, 116,25 in 265 µg/mL. Kljub zmanjšanju bakterijske rasti, je uporaba ionov omejena, saj citotoksično delujejo na celice mišjih fibroblastov, izjema so le SiO2 ioni. Pri E. coli je MIK Ag100 nanodelcev med 3,75 in 7,5 mg/mL, Ag20-30 nanodelcev med 1 in 3,75 mg/mL, pri ZnO in Cu nanodelcih pa 3,75 mg/mL. MIK SiO2 nanodelcev je 7,5 mg/mL za E. coli. Pri S. aureus znašajo MIK Ag100, Ag20-30 in ZnO nanodelcev 3,75 mg/mL, pri Cu nanodelcih 1 mg/mL ter pri SiO2 nanodelcih 7,5 mg/mL. Rezultati dokazujejo protibakterijske učinke ionov in kovinskih nanodelcev na bakterije S. aureus in E. coli.

Language:Slovenian
Keywords:nanodelci, kovinski nanodelci, kovinski ioni, protibakterijska učinkovitost, Escherichia coli, Staphylococcus aureus, spektroskopske meritve optične gostote, štetje kolonij na trdem gojišču, minimalna inhibitorna in baktericidna koncentracija
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Publisher:[M. Semič]
Year:2021
PID:20.500.12556/RUL-124143 This link opens in a new window
UDC:620.3:579.6:615.281
COBISS.SI-ID:48520451 This link opens in a new window
Publication date in RUL:07.01.2021
Views:1186
Downloads:143
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Secondary language

Language:English
Title:Comparison of the antimicrobial efficiency of metal nanoparticles and their soluble ionic part on the bacteria Staphylococcus aureus in Escherichia coli
Abstract:
Commercially available Ag100, Ag20-30, ZnO, Cu and SiO2 nanoparticles were used in the practical part of the master’s thesis. We studied the antibacterial effects of metal nanoparticles and released ions. The shape of the nanoparticles was characterized by transmission electron microscopy (TEM). The effect of ions and nanoparticles on bacteria Escherichia coli in Staphylococcus aureus was determined by spectroscopic measurements of optical density (OD600) and colony forming unit method (CFU). The effect of ions on humane cells was determined by cytotoxic test and the exact concentration of ions released from nanoparticles was determined by inductively coupled mass spectroscopy (ICP-MS). The effect of metal nanoparticles on morphology of bacterial cells in biofilms was examined by scanning electron microscopy (SEM). Minimal inhibitory concentration (MIC) of Ag20-30 ions is between 0,64 and 0,85 µg/mL, for E. coli. MICs of Ag20-30 and Cu ions are 0,85 and 265 µg/mL, for S. aureus. Despite antimicrobial efficacy of metal ions, the use is limited because they have cytotoxic effect on mouse fibroblast cells, except SiO2 ions. MIC of Ag100 NPs is between 3,75 and 7,5 mg/mL, MIC of Ag20-30 NPs is between 1 and 3,75 mg/mL, MICs of Cu and ZnO NPs are 3,75 mg/mL and MIC of SiO2 NPs is 7,5 mg/mL, for E. coli. MICs of Ag100, Ag20-30 and ZnO NPs are 3,75 mg/mL, MIC of Cu NPs is 1 mg/mL and MIC of SiO2 NPs is 7,5 mg/mL, for S. aureus. The results prove the antibacterial effects of ions and nanoparticles on bacteria S. aureus and E. coli.

Keywords:nanoparticles, metal nanoparticles, metal ions, antibacterial efficacy, Escherichia coli, Staphylococcus aureus, spectroscopic measurements of optical density, colony forming unit, minimal inhibitory concentration, minimal bactericidal concentration

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