Interakcije egerolizinov iz gliv rodu Pleurotus z umetnimi in biološkimi membranami

Panevska, Anastasija (author), Sepčič, Kristina (mentor)

Egerolizini iz gliv ostrigarjev ostreolizin A6 (OlyA6), pleurotolizin A2 (PlyA2) in erilizin A (EryA) interagirajo s ceramid fosfoetanolaminom (CPE), membranskim lipidom specifičnim za nevretenčarje, še posebej za žuželke. Omenjeni lipid se v membranah višjih organizmov vključno s sesalci nahaja v sledovih ali ga sploh ni. Egerolizini, skupaj s proteinskim partnerjem pleuorotolizinom B (PlyB), ki ga proizvaja ista goba, tvorijo transmembranske pore in posledično delujejo toksično na žuželke, ki v svojih membranah vsebujejo omenjeni receptorski lipid. Takšni proteinski kompleksi (egerolizinski proteini + proteinski partner PlyB) kažejo toksičen učinek proti ličinkam dveh, v ekonomskem smislu, najbolj pomembnih rastlinskih škodljivcev - koruznega in koloradskega hrošča. Toksičnost omenjenih proteinskih kompleksov je primerljiva s toksičnostjo proteinskih kristalnih toksinov (proteinov Cry). Omenjene proteine bi lahko uporabili kot biopesticide za zatiranje koloradskega in koruznega hrošča.

• Language: Slovenian
• Keywords: egerolizini, porotvorni proteini, ceramid fosfoetanolamin, biopesticidi
• Work type: Doctoral dissertation
• Organization: MF - Faculty of Medicine
• Year: 2020
• PID: 20.500.12556/RUL-124114
• COBISS.SI-ID: 53607427
• Publication date in RUL: 01.01.2021

Secondary language

• Language: English
• Title: Interaction of aegerolysins from fungal genus Pleurotus with artificial and biological membranes

Abstract

Aegerolysins from the oyster mushroom ostreolysin A6 (OlyA6), pleurotolysin A2 (PlyA2) and erylysin A (EryA) interact with the ceramide phosphoethanolamine (CPE), a membrane lipid specific to invertebrates, especially insects. This lipid is found only in trace amounts, or is completelly absent in the membranes of higher organisms, including mammals. Aegerolysins, together with their partnering protein pleurotolysin B (PlyB) produced by the same mushroom, form transmembrane pores, and therefore have a toxic effect on insects that contain the lipid receptor in their membranes. Such protein complexes (aegerolysin proteins + PlyB) show a toxic effect against larvae of the two economically most important plant pests - the Western Corn rootworm and Colorado potato beetle. The toxicity of these protein complexes is comparable to the toxicity of proteinaceous crystal toxins (Cry proteins). These proteins could be used as biopesticides to control the Colorado potato beetle and Western Corn rootworm.

• Keywords: aegerolysins, pore-forming proteins, ceramide phosphoethanolamine, biopesticides