Nitrosative stress in the frontal cortex from dogs with canine cognitive dysfunction

Prpar Mihevc, Sonja (author), Zakošek, Maja (author), Štrbenc, Malan (author), Rogelj, Boris (author), Majdič, Gregor (author)

Canine cognitive dysfunction (CCD) is an age-related disorder similar to human Alzheimer's disease (AD) that occurs in elderly dogs. Nitrosative stress has been implicated as one of the causes leading to neurodegenerative diseases, particularly AD. Its involvement in the development of CCD has not been studied so far. In the present study, immunohistochemical staining detected all three isoforms of nitric oxide synthases (nNOS, eNOS, and iNOS) and 3-nitrotyrosine (3-NT) in brains from CCD-affected dogs and non-demented control dogs in all layers of the canine frontal cortex. In CCD-affected and non-demented brains, nNOS was highly expressed in pyramidal-like neurons in the upper cortical layers. nNOS has also been observed in astrocytes in the CCD frontal cortex. The nNOS immunohistochemical staining was statistically significantly elevated in dogs with CCD in comparison to non-demented dogs. Blood vessel wall cells were positive for eNOS, which was also expressed in astrocytes and neurons. Intense 3-NT immunoreactivity was observed in the upper cortical layers, where amyloid-beta deposits spread in the last stage of CCD. Brain cells in the same area were highly immunoreactive for iNOS. This infers that neuroinflammation and nitrosative stress might exacerbate the neurodegenerative process in CCD-affected brains, ultimately leading to cognitive impairment

• Language: English
• Keywords: Canine cognitive dysfunction, nitrosative stress, nNOS, eNOS, iNOS, 3-nitrotyrosine
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: art. 573155, str. 1-14
• Numbering: Vol. 7
• PID: 20.500.12556/RUL-122068
• UDC: 636.7.09:616.89
• ISSN on article: 2297-1769
• DOI: 10.3389/fvets.2020.573155
• COBISS.SI-ID: 38304259
• Publication date in RUL: 19.11.2020

Record is a part of a journal

• Title: Frontiers in veterinary science
• Shortened title: Front. vet. sci.
• Publisher: Frontiers Media
• ISSN: 2297-1769
• COBISS.SI-ID: 3969402

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 19.11.2020

Secondary language

• Language: Undetermined
• Keywords: Pasja kognitivna motnja, nitrozativni stres, nNOS, eNOS, iNOS, 3-nitrotirozin

Projects

• Funder: ARRS - Slovenian Research Agency
• Funding programme: Evropski sklad za regionalni razvoj, Raziskovalci na začetku kariere 2.0
• Project number: P4-0053
• Name: Razvoj in uporaba naprednih celičnih terapij za zdravljenje demence pri psih
• Acronym: C3330-17-529031