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Vpliv števila cepljenj proti steklini in starosti psov na nivo specifičnih protiteles proti virusu stekline
ID Kulić, Nina (Author), ID Narat, Mojca (Mentor) More about this mentor... This link opens in a new window, ID Malovrh, Tadej (Comentor)

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Abstract
Steklina je virusna bolezen osrednjega živčnega sistema in je zoonoza. Najučinkovitejši način za preprečevanje urbane stekline je preventivno cepljenje psov, ki morajo biti v Sloveniji cepljeni s prvim odmerkom med 12. in 16. tednom starosti, z drugim in tretjim odmerkom v razmiku 12 mesecev od predhodnega, naslednja cepljenja pa se izvedejo v skladu z navodili proizvajalca cepiva. Cilj naloge je bil izračun korelacije med starostjo psov in nivojem protiteles, saj bi pozitivna korelacija lahko pomenila doprinos k spremembi shem cepljenja. Za določanje titra specifičnih nevtralizacijskih protiteles proti virusu stekline smo uporabili test FAVN. Za zaščito pred okužbo z virusom stekline je potreben titer protiteles ⡥ 0,5 IE/ml. V vseh državah, iz katerih smo imeli vzorce in smo jih pregledali, je bil delež pozitivnih vzorcev višji od deleža negativnih. Največji delež negativnih vzorcev je pripadal psom, mlajšim od enega leta, ki so bili cepljeni le enkrat. Le pri vzorcih iz Srbije in Črne gore smo opazili dovolj veliko pozitivno korelacijo med starostjo psov in višino titra protiteles, da lahko rečemo, da starost psa vpliva na višino titra protiteles. Pri ostalih državah korelacije nismo opazili, zato obstoječe sheme prvih treh cepljenj ne bi spreminjali. Za nadaljnja cepljenja predlagamo, da se opravijo v razmiku od enega do treh let od predhodnega cepljenja.

Language:Slovenian
Keywords:zoonoza, virus stekline, cepljenje, cepljenje psov, mikrobiološka diagnostika, protitelesa, test FAVN, titer nevtralizacijskih protiteles
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Publisher:[N. Kulić]
Year:2020
PID:20.500.12556/RUL-121989 This link opens in a new window
UDC:578.824:614.47:636.7:616-079
COBISS.SI-ID:37851395 This link opens in a new window
Publication date in RUL:14.11.2020
Views:1169
Downloads:152
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Secondary language

Language:English
Title:The influence of number of anti-rabies regular vaccinations and dog age on the level of specific antibodies
Abstract:
Rabies is a zoonotic viral disease that attacks the central nervous system. The best way to prevent urban rabies is preventive vaccination of dogs. Dogs in Slovenia must be vaccinated with the first dose in the age between 12 and 16 weeks and with the second and third dose 12 months after the prior one. All the following vaccinations are given in accordance with vaccine manufacturer´s instructions. The aim of this study was to calculate the correlation between the age of dogs and the titer of antibodies, since positive correlation might contribute to the change of the vaccination scheme. We evaluated rabies neutralising antibodies titers via FAVN. Antibody titres ⡥ 0,5 IE/ml are considered protective against rabies infection. Among all tested samples belonging to different countries the majority was positive and among negative samples the majority was entitled to dogs younger than a year, that were vaccinated only once. Only in the case of Serbia and Montenegro there was a big enough positive correlation between the age of dogs and the titre of antibodies, to say that the age of dogs influences the titre of antibodies. In the case of other countries there was no correlation, therefore we would not change the existing scheme of the first three vaccinations. We suggest that all the following vaccinations are carried out one to three years after the prior dose.

Keywords:zoonosis, rabies virus, vaccination, vaccination of dogs, microbiology diagnosis, antibodies, FAVN test, neutralising antibody titer

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