Protiglivna aktivnost vodnih in organskih izvlečkov izbranih vrst pelinov

Miljuš, Staš (author), Strgulc Krajšek, Simona (mentor), Anžlovar, Sabina (comentor)

V magistrskem delu smo testirali protiglivno aktivnost etanolnih in vodnih izvlečkov, pridobljenih iz listov, korenin in socvetij treh vrst pelina (Artemisia verlotiorum, A. vulgaris, A. annua). Protiglivno aktivnost smo testirali z difuzijsko metodo na agarju proti vrstam: Aspergillus flavus, Alternaria infectoria, Fusarium poae, Fusarium oxysporum, Botrytis cinerea in Alternaria alternata. Zaviranje rasti gliv se je med posameznimi izvlečki močno razlikovalo, prav tako se je razlikoval učinek izvlečkov na posamezno glivo. Izvlečki so rast določenih gliv tudi vzpodbudili. Etanolni izvleček listov A. verlotiorum je edini pokazal protiglivno delovanje na vseh testiranih glivah. Rast gliv je najbolj zaviral etanolni izvleček listov vrste A. annua, ki je bil v večini primerov primerljiv s širokospektralnim fungicidom azoxystrobinom. Razlik v delovanju izvlečkov podzemnih in nadzemnih rastlinskih delov ni. Razlike v delovanju etanolnih in vodnih izvlečkov so se pokazale pri izvlečkih A. verlotiorum, ne pa tudi pri A. vulgaris. Med testiranimi rastlinskimi vrstami so imeli izvlečki A. annua največji protiglivni učinek, ki je bil primerljiv z protiglivnim delovanjem azoxystobina. Izvlečki A. annua imajo tako največji potencial za uporabo kot biofungicidi v živilski industriji.

• Language: Slovenian
• Keywords: Artemisiap, protiglivno delovanje, fungicidna aktivnost, vodni izvlečki, etanolni izvlečki
• Work type: Master's thesis/paper
• Organization: BF - Biotechnical Faculty
• Year: 2020
• PID: 20.500.12556/RUL-121690
• COBISS.SI-ID: 44390915
• Publication date in RUL: 23.10.2020

MILJUŠ, Staš, 2020, Protiglivna aktivnost vodnih in organskih izvlečkov izbranih vrst pelinov [online]. Master’s thesis. [Accessed 24 April 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=121690

Secondary language

• Language: English
• Title: Antifungal activity of organic and aqueous extracts from selected species of Artemisia

Abstract

In the master thesis we tested antifungal activity of ethanol and aqueous extracts, derived from roots, flowers and leaves of three species of Artemisia (Artemisia verlotiorum, A. vulgaris, A. annua). Antifungal activity was tested with the diffusion method on agar on six different fungal species (Aspergillus flavus, Alternaria infectoria, Fusarium poae, Fusarium oxysporum, Botrytis cinerea and Alternaria alternata). Fungus growth inhibition was differing among different extracts. Extracts have also accelerated the growth of some fungi. Ethanol leaf extract from A. verlotiorum was the only one that had a growth inhibitory effect on all tested fungi. Leaf ethanol extract from A. annua has shown the most antifungal activity and was also comparable with wide spectrum synthetic fungicide azoxystrobin. There were observed differences between extracts from underground and aboveground plant parts. Differences in antifungal activity between ethanol and aqueous extracts were shown for A. verlotiorum, but not A. vulgaris. Among all tested plant species extracts from A. annua have shown the biggest growth inhibitory effects on tested fungi, that were also comparable with azoxystrobin. Therefore A. annua has the most potential as a biological fungicide and for uses in the food industry.

• Keywords: Artemisia, Antifungal activity, ethanol extracts, aqueous extracts