Določanje koncentracije antikoagulanta v epruvetah za vakuumski odvzem krvi

Dragan, Jure (author), Gros, Nataša (mentor)

Raztopine natrijevih soli citronske kisline in/ali puferne raztopine natrijevega citrata (kombinacija citronske kisline in njenih natrijevih soli) so v kliničnih laboratorijih pogosto uporabljene raztopine, ki inhibirajo strjevanje krvi. Citratni pufer (antikoagulant) v koagulacijskih epruvetah za vakuumski odvzem krvi, tako sestavljajo različne oblike citrata, ki otežujejo njegovo določanje. V okviru magistrskega dela smo optimizirali postopek za določanje skupne koncentracije citratnih oblik v vodnih raztopinah in spremljali kakovost izbranih vakuumiranih epruvet v času roka njihove uporabnosti. Ustreznost vakuumiranih epruvet smo preverjali z dvema parametroma in sicer z določanjem vsesanega volumna epruvet in določanjem koncentracije citrata (antikoagulanta) v njih. Vsesani volumen ovrednotenih epruvet je v času roka trajanja njihove uporabnosti ustrezal specifikacijam in ni presegel ± 10 % predpisane vrednosti. Po pričakovanjih je vsesani volumen vakuumiranih epruvet tekom njihovega vrednotenja postopno padal, kar pripisujemo zmanjševanju podtlaka v epruvetah. V skladu s pričakovanji smo ob padcu vsesanega volumna epruvet opazili naraščanje koncentracije antikoagulanta.

• Language: Slovenian
• Keywords: določanje koncentracije antikoagulanta, epruvete za vakuumski odvzem krvi, natrijev citrat
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2020
• PID: 20.500.12556/RUL-121166
• COBISS.SI-ID: 37052931
• Publication date in RUL: 30.09.2020

Secondary language

• Language: English
• Title: Determining anticoagulant concentration in evacuated blood-collection tubes

Abstract

Solutions of citric acid sodium salts and/or sodium citrate buffer solutions (combination of citric acid and its sodium salts) are often used in clinical laboratories as solutions that inhibit coagulation of blood. Citrate buffer (anticoagulant) in evacuated blood collection tubes consists of different forms of citrate. The determination of citrate is therefore difficult. In this work we optimized the method for the determination of the total concentration of citrate species in aqueous solutions and monitored the quality of selected evacuated blood collection tubes during their shelf life. The suitability of the evacuated blood collection tubes was determined by evaluating two parameters, the draw volume measurement and the determination of the citrate (anticoagulant) concentration. The draw volume of evaluated tubes did not exceed ± 10 % of the indicated value during their shelf life and thus met the specifications. As expected, the draw volume of the evacuated blood collection tubes decreased gradually during their evaluation due to the reduction of the vacuum in the tubes. An increase in the anticoagulant concentration was observed as the draw volume of the tubes decreased.

• Keywords: determining anticoagulant concentration, evacuated blood collection tubes, sodium citrate