SODOBNI ANALIZNI PRISTOPI PRI KARAKTERIZACIJI PROPOLISA

Deučman, Špela Katarina (author), Kolar, Mitja (mentor)

Propolis je naravna smolnata zmes, ki jo proizvajajo čebele delavke (Apis mellifera). Običajno je sestavljen iz 50 % smole, 30 % voska, 10 % eteričnih olj, 5 % cvetnega prahu in 5 % drugih snovi. Ima mnoge biološke učinke med drugim antibakterijske, protiglivne in antioksidativne. Različne vzorce surovega propolisa smo ekstrahirali s 70 % (v/v) etanolom in njihovo sestavo analizirali s HPLC-DAD in HPLC-ECD. Na podlagi HPLC rezultatov in nadaljnje PCA analize smo izbrali etanolne ekstrakte propolisa za mikrobiološko analizo. Z mikrodilucijsko metodo smo določili minimalne inhibitorne koncentracije proti bakterijam vrste Staphylococcus aureus in Escherichia coli ter proti kvasovki vrste Candida albicans. Izvedeni sta tudi bili termogravimetrična analiza in diferenčna dinamična kalorimetrija na vzorcih surovega rjavega in rdečega propolisa ter čebeljega voska.

• Language: Slovenian
• Keywords: propolis, HPLC, mikrodilucijska metoda, TGA, DSC
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2020
• PID: 20.500.12556/RUL-119669
• COBISS.SI-ID: 30011907
• Publication date in RUL: 10.09.2020

Secondary language

• Language: English
• Title: NOVEL ANALYTICAL APPROACHES IN THE CHARACTERIZATION OF PROPOLIS

Abstract

Propolis is a resin-like mixture produced naturally by worker bees (Apis mellifera). It usually consists of 50 % resin, 30 % wax, 10 % essential oils, 5 % pollen and 5 % of various other substances. Besides many biological effects, it has antibacterial, antifungal and antioxidative properties. We extracted samples of raw propolis with 70% (v/v) ethanol and then analysed its composition with HPLC-DAD and HPLC-ECD. Based on the results of HPLC and PCA analysis, we selected ethanol propolis extract samples for microbiological analysis. Using the microdilution method, we determined minimal inhibitory concentrations of the samples on the bacterial species Staphylococcus aureus and Escherichia coli as well as on the yeast species Candida albicans. For samples of raw brown, red propolis and beeswax we also preformed thermogravimetric analysis together with differential scanning calorimetry.

• Keywords: propolis, HPLC, microdilution method, TGA, DSC