Ultra-high-pressure liquid chromatography was developed in laboratories in the late 1990s, with the aim of achieving rapid high-resolution analysis predominantly on capillary columns filled with 1–1.7 micron particles. UHPLC is widely used in analytical chemistry for the separation, identification and quantitative analysis of compounds. It provides us with high speed, high resolution, sensitivity and short lead time. This requires very high pressures of up to 1500 bar and columns filled with particles smaller than 2 microns. We can divide them in two groups, into hybrid and silica-based particles. The advantages of UHPLC systems are the use of less organic solvents and a reduction in generated waste. UHPLC systems differ from each other in maximum pressure drop, different flow rates, dead volume and many other properties. Nowadays, UHPLC columns are used in environmental, food and chemical analyzes, where it is necessary to analyze a large number of samples in the shortest possible time. It is also widely used in pharmaceutical companies during the discovery and development of new drugs due to its rapid separation and reduced costs.
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