Uporaba raziskovalnega invertnega mikroskopa za fluorescenčno termometrijo

Mušič, Jure (author), Golobič, Iztok (mentor), Zupančič, Matevž (co-mentor)

V delu obravnavamo uporabo raziskovalnega invertnega mikroskopa Nikon Eclipse Ti2 za fluorescenčno termometrijo. V okviru testiranja invertnega mikroskopa za uporabo za fluorescenčno termometrijo smo izvedli eksperiment merjenje fluorescence vzorcev. Merilni sistem je zastavljen tako, da omogoča hkratno uporabo optične pincete in dveh kamer z globalno in vrtečo zaslonko. Na primeru spektra fluorescenčnih vzorcev smo prikazali delovanje invertnega mikroskopa, vzbujevalnega LED svetila, tekočekristalnega filtra in kamere z vrtečo zaslonko in CMOS senzorjem.

• Language: Slovenian
• Keywords: fluorescenca, fluorescenčna termometrija, invertni mikroskop, meritve spektra fluorescence
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FS - Faculty of Mechanical Engineering
• Place of publishing: Ljubljana
• Publisher: [J. Mušič]
• Year: 2020
• Number of pages: XXII, 52 str.
• PID: 20.500.12556/RUL-119346
• UDC: 519.6:536.51(043.2)
• COBISS.SI-ID: 32531715
• Publication date in RUL: 08.09.2020

Secondary language

• Language: English
• Title: Use of research inverted microscope for fluorescence thermometry

Abstract

This work discusses the use of the Nikon Eclipse Ti2 research inverted microscope for fluorescence thermometry. As part of the testing of the inverted microscope for use in fluorescence thermometry, we performed an experiment measuring the fluorescence of samples. The system is designed to allow the simultaneous use of optical tweezers and two cameras with global and rolling shutter. In the case of the spectrum of fluorescent samples, we showed the operation of an inverted microscope, an excitation LED lamp, a liquid crystal filter and a rolling shutter camera with CMOS sensor.

• Keywords: fluorescence, fluorescence thermometry, inverted microscope, fluorescence specter mesurments