Proteomic analysis of fresh and liquid-stored boar spermatozoa

Premrov Bajuk, Blanka (author), Zrimšek, Petra (author), Zakošek, Maja (author), Tilocca, Bruno (author), Soggiu, Alessio (author), Bonizzi, Luigi (author), Roncada, Paola (author)

In this study comparative proteomics was used to define changes in the expression of the spermatozoa proteins during liquid storage. Semen from eight boars was analyzed on the day of collection and after liquid preservation at 15–17 °C for three days. Sperm parameters (concentration, motility, morphology, vitality) and percentage of non-capacitated and acrosomal-reacted spermatozoa were determined. Sperm proteins were extracted and separated by two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and proteomic profiles were computationally compared to highlight differentially expressed protein spots that were, in turn, identified by mass spectrometry. The intensities of four spots were significantly different between fresh and liquid stored sperm. Namely: ATP citrate lyase, chaperonin containing T-complex polypeptide 1 (TCP1) subunit ε and probable phospholipid-transporting ATP-ase were over-expressed in liquid stored sperm, whereas cytosolic non-specific dipeptidase was over-expressed in fresh sperm. These differentially expressed proteins could be used as plausible biomarkers for the evaluation of boar semen quality and spermatozoa survival after liquid storage and could help to address problems associated with sperm preservation.

• Language: English
• Keywords: proteomics, spermatozoa, metabolism, semen analysis, semen preservation, methods, liquid stored semen, boar spermatozoa, swine
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 10 str.
• Numbering: Vol. 10, iss. 4, art. 553
• PID: 20.500.12556/RUL-118011
• UDC: 636.4.082.453.5:612.2:591.463.1:542.816
• ISSN on article: 2076-2615
• DOI: 10.3390/ani10040553
• COBISS.SI-ID: 5144442
• Publication date in RUL: 11.08.2020

Record is a part of a journal

• Title: Animals
• Shortened title: Animals
• Publisher: MDPI AG
• ISSN: 2076-2615
• COBISS.SI-ID: 519120409

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.04.2020

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0053
• Name: Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

• Funder: EC - European Commission
• Funding programme: Horizon 2020
• Project number: FA 1002