Vrednotenje stabilnosti kanabidiola v kozmetičnih izdelkih z merjenjem njegove vsebnosti

Bevec, Renata (author), Kreft, Samo (mentor), Tavčar Benković, Eva (comentor)

Kanabidiol (CBD) je eden od kanabinoidov, najdenih v rastlini konoplji (Cannabis sativa L.). Ima relativno nizko toksičnost, povzroča malo znanih neželenih učinkov in ker ni psihoaktiven, ni uvrščen med prepovedane droge. Kanabinoide se vedno več uporablja v zdravilih, kozmetiki in prehranskih dopolnilih. Baza kozmetičnih sestavin Evropske komisije, CosIng, CBD navaja v svojem seznamu sestavin, ki so dovoljene za kozmetično uporabo. Kozmetični izdelki morajo biti varni, kakovostni in učinkoviti. Na njihovo kakovost vpliva tudi stabilnost, ki mora biti zagotovljena skozi celotno obdobje uporabe. CBD dobro prehaja skozi kožo, zato je možna uporaba mazil in transdermalnih obližev. CBD ima nevrozaščitni, antiepileptični, antipsihotični, analgetični, protivnetni, in protitumorni učinek. V Evropi in Ameriki je odobrena zdravilna učinkovina CBD in se uporablja v zdravilih z dovoljenjem za promet Sativex® in Epidiolex®. V magistrski nalogi smo obravnavali stabilnost CBD v kozmetičnih izdelkih. Spremljali smo vpliv pogojev: svetlobe in temperature (4 °C, 25 °C in 40 °C), vpliv vsebnikov (plastična epruveta, plastičen lonček, brezzračna črpalka in aluminijasta tuba) in vpliv formulacij (krema za obraz brez in s konzervansom, krema za roke, regenerativna krema). Vsebnost CBD smo v obdobju treh mesecev ugotavljali s tekočinsko kromatografijo visoke ločljivosti. Pri spremljanju vpliva temperature smo ugotovili, da razpad CBD s temperaturo narašča. V plastični epruveti je bil pri vzorcih brez konzervansa najmanjši razpad CBD pri 4 °C: 2,2 %, največji razpad pa pri 40 °C, in sicer, 14,1 %. Iz rezultatov je razvidno tudi, da ima svetloba vpliv na razpad CBD. Večji razpad CBD je bil viden pri vzorcih, izpostavljenih svetlobi. Pri primerjavi vsebnikov se je za najboljšega izkazala aluminijasta tuba, ki ne prepušča svetlobe in zraka. Med vsemi formulacijami pa je bil CBD najbolj stabilen v kremi za obraz, kjer je bil razpad po treh mesecih 2,9 %, sledila je krema za roke z razpadom 4,6 %, za najslabšo pa se je izkazala regenerativna krema, kjer je razpadlo 13,2 % CBD. Pri vseh vzorčenjih se je prisotnost konzervansa naravnega izvora izkazala za negativno, saj je bila stabilnost CBD slabša ob njegovi prisotnosti.

• Language: Slovenian
• Keywords: CBD, kanabidiol, stabilnost, kozmetični izdelki, vsebnost
• Work type: Master's thesis/paper
• Organization: FFA - Faculty of Pharmacy
• Year: 2020
• PID: 20.500.12556/RUL-117352
• Publication date in RUL: 08.07.2020

Secondary language

• Language: English
• Title: Evaluation of cannabidiol stability in cosmetic products by measuring its content

Abstract

Cannabidiol (CBD) is one of the cannabinoids found in the cannabis plant (Cannabis sativa L.). It has relatively low toxicity, causes little known side effects and is not psychoactive and therefore not enlisted among illicit drugs. Cannabinoids are used in medicines, cosmetics and nutritional supplements. In the European Commission's cosmetic ingredients database, CosIng, CBD is authorized as approved cosmetic substance. Cosmetic products must be safe, of good quality and effective. Their quality is also affected by stability, which must be ensured throughout the period of use. CBD passes well through the skin, so ointments and transdermal patches are possible. CBD has neuroprotective, antiepileptic, antipsychotic, analgesic, anti-inflammatory and antitumor properties. Europe and United States of America have approved Sativex® and Epidiolex®, which contain CBD as an active pharmaceutical ingredient. In Master thesis we evaluated the stability of CBD in cosmetic products. We monitored the effect of conditions: light and temperature (4 °C, 25 °C, 40 °C), containers (plastic tube, plastic pot, airless pump and aluminum tube) and formulations (preservative and preservative-free face cream, hand cream and regenerative cream). The CBD content was determined over a three-month period by high-performance liquid chromatography. In monitoring the effect of temperature, we found that the decay of CBD increased with temperature. In the plastic tube, the minimum decay of CBD at 4 °C was 2.2% for the preservative free-face cream, and the highest decay was at 40 °C, namely 14.1%. The results show that light has an effect on the decay of CBD. Greater decay of CBD was seen in samples exposed to light. When comparing the containers, the aluminum tube, which does not transmit light and air, proved to be the best. According to obtained results, most stable formulations were found in preservative-free face cream, with a decay of 2.9% after three months, followed by hand cream with a decay of 4.6%, and the worst was the regenerative cream, where it decomposed 13.2% of CBD. We conclude, that the presence of a preservative of natural origin, was found to be negative, as the stability of CBD was impaired in its presence.

• Keywords: CBD, cannabidiol, stability, cosmetic products, content