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Določanje vitamina E v rastlinskih oljih z diferenčno pulzno voltametrijo
ID Toplak, Blaž (Author), ID Kolar, Mitja (Mentor) More about this mentor... This link opens in a new window

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Abstract
Kontrola kakovosti olj in maščob se dnevno izvaja v prehrambeni kemiji in industriji. Vsebnost antioksidantov v rastlinskih oljih je zelo pomembna pri določanju stabilnosti izdelkov. Vitamin E sodi v eno največjih skupin naravnih antioksidantov, ki vključuje več tokoferolov in tokotrienolov. Za te naravne fenolne spojine je značilno, da so elektrokemijsko aktivne. V diplomski nalogi sem določal vitamin E z uporabo elektrokemijske tehnike - diferenčne pulzne voltametrije (DPV) v sončničnem, ekstra deviškem oljčnem in konopljinem olju. Metodo sem najprej optimiral z določanjem najustreznejših reakcijskih pogojev. Po določitvi optimalnih pogojev sem vitamin E iz vzorcev rastlinskih olj ekstrahiral z dvema različnima postopkoma - prvi je bil postopek direktne ekstrakcije vitamina E v absolutni etanol, drugi pa je vseboval saponifikacijo olja in nadaljnjo ekstrakcijo. Za določanje sem uporabil metodo direktne ekstrakcije, ker se je izkazala za ustreznejšo in dobro ponovljivo. Slednjega pri metodi s saponifikacijo nisem dosegel. Določanje vitamina E v rastlinskih oljih z diferenčno pulzno voltametrijo na elektrodi iz steklastega ogljika(GCE) se je izkazalo za dobro in učinkovito pri oljih z višjo koncentracijo α-tokoferola.

Language:Slovenian
Keywords:elektrokemija, voltametrija, diferenčna pulzna voltametrija, vitamin E
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Technology
Year:2020
PID:20.500.12556/RUL-115986 This link opens in a new window
COBISS.SI-ID:13931011 This link opens in a new window
Publication date in RUL:05.05.2020
Views:1424
Downloads:214
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Secondary language

Language:English
Title:Determination of Vitamin E in vegetable oils using differential pulse voltammetry
Abstract:
Quality control of oils and fats is important in food chemistry and in the food industry. The content of antioxidant substances in vegetable oil products is of great importance for determining the stability of the products. Vitamin E represents one of the largest groups of natural antioxidants, including several tocopherols and tocotrienol compounds. These phenolic compounds are among the many naturally occurring substances known to be electrochemically active. In my work, I have determined vitamin E using differential pulse voltammetry (DPV). I have specified vitamin E in sunflower oil, extra virgin olive oil and hemp oil. First I optimized the method by determining optimal conditions. Then I tried to extract vitamin E from vegetable oil samples using two different techniques. The first was the method of direct extraction of vitamin E in absolute ethanol and the second involved saponification of the oil and further extraction. For the determination of vitamin E, I used the method of direct extraction because it was repeatable. I did not achieve this with the saponification method. The determination of vitamin E by differential pulse voltammetry has proven to be an effective method in oils with a higher concentration of α- tocopherol.

Keywords:electrochemistry, voltammetry, differential pulse voltammetry, Vitamin E

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