In the master's thesis we tested antibiotic susceptibility of staphylococci, isolated from goat's milk, to the veterinary most used antibiotics (penicillin, cephalothin, cefoxitin, enrofloxacin, fusidic acid, erythromycin, clindamycin, gentamicin, nitrofurantoin, tetracycline and thrimethoprim/sulfamethoxazole). We analysed 32 staphylococci from milk of organic and 40 strains from milk of conventional goat farming. In general, coagulase negative staphylococci (44 strains) dominated over coagulase positive staphylococci (28 strains, S. aureus). Antibiotic susceptibility testing was performed using microdilution method. All staphylococci were sensitive to cephalothin, gentamicin and nitrofurantoin, while staphylococci from organic farming were additionaly sensitive to fusidic acid. Among staphylococci, the most sensitive was one strain of S. warneri species. 80 % of staphylococci were resistant to trimethoprim/sulfamethoxazole, and 20 % to tetracycline and penicillin. Strains of S. aureus were most resistant to trimethoprim/sulfamethoxazole and 7 % to penicillin, while all S. aureus strains were sensitive to enrofloxacin, fusidic acid, cephalothin, nitrofurantoin and gentamicin. More than 80 % coagulase negative staphylococci were resistant to trimethoprim/sulfamethoxazole and 20 % to penicillin. Type of farming (conventional or organic) did not significantly affected resistance of tested staphylococci, isolated from goat's milk.
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