Immunoproteasome is a part of well-controled ubiquintin-proteasome system, that is expressed mainly in immune cells and collaborates in degredation of damaged proteins. In this way it significantly contributes to the maintenance of normal cell homeostasis balance. Its inhibition is considered to be a well-established therapy for many avtoimmune, cancer and neurodegenerative diseases. The purpose of this master thesis was to develop an UHPLC analytical method to determine the intracelullar concentration of PR957 and DPLG3, peptide type imunoproteasome inhibitors. There was a lack of effect on cells and the reason for this may be too small intracellular concentration of both, because of e.g. degradation or difficulty in crossing plasma membrane. The determination of intracellular concentrations may help determine the reason for the lack of effect.
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