Biokemijske in optične značilnosti Hostovega kamnokreča (Saxifraga hostii) v raznolikih razmerah sevanja UV- B na višinskem gradientu Kobariškega Stola

Rovtar, Jerneja (author), Trošt Sedej, Tadeja (mentor)

Preučevali smo vpliv nadmorske višine in UV-B sevanja na biokemijske in optične lastnosti listov Hostovega kamnokreča (Saxifraga hostii). Hostov kamnokreč je alpinska zelnata trajnica z ozkimi listi, nameščenimi v rozeto. Na prisojnem pobočju Kobariškega Stola smo postavili plošče z in brez UV filtrov na dveh nadmorskih višinah: 1095 m. n. m. in 1504 m. n. m. Rastline smo spremljali tekom celotne rastne sezone, vzorce smo nabrali junija, avgusta in septembra. V obdobju med prvim in zadnjim vzorčenjem smo merili tudi temperaturo tal. V laboratoriju smo izmerili vsebnost klorofila a in b, karotenoidov, antocianov ter UV-absorbirajočih snovi. Merili smo tudi odbojnost in presevnost listov v območju 280-710 nm. S pomočjo enosmerne ANOVE smo primerjali vpliv različne temperature in različnega UV sevanja na rastline ter ugotavljali ali se podatki statistično značilno razlikujejo. Rezultate smo primerjali znotraj enega vzorčenja. Z dvosmerno ANOVO smo ugotavljali vpliv temperature in UV sevanja ter interakcije med njima na posamezne biokemijske in optične značilnosti izbrane rastline. Ugotovili smo, da opazovane okoljske razmere niso vplivale na vsebnost UV-absorbirajočih snovi, saj je rastlina prilagojena na razmere s povišanim UV sevanjem. Temperatura in UV sevanje v avgustu ne vplivata na klorofile, karotenoide in antociane, v septembru pa vplivata, kar nakazuje zgodnejša senescenca. UV sevanje je v interakciji z drugimi okoljskimi dejavniki. Rastline, izpostavljene različnim okoljskim razmeram na dveh različnih nadmorskih višinah, se različno odzivajo na UV sevanje.

• Language: Slovenian
• Keywords: biokemijske lastnosti, ekologija, Hostov kamnokreč, optične lastnosti, UV-B sevanje
• Work type: Master's thesis/paper
• Organization: BF - Biotechnical Faculty
• Year: 2019
• PID: 20.500.12556/RUL-113184
• COBISS.SI-ID: 5250639
• Publication date in RUL: 11.12.2019

Secondary language

• Language: English
• Title: Biochemical and leaf optical properties of Saxifraga hostii in diverse conditions of UV-B radiation and altitude gradient on Kobariški Stol

Abstract

We studied the effect of altitude and UV-B radiation on biochemical traits and optical leaf properties of Saxifraga hostii. It is alpine perennial plant with narrow leaves forming rosette. We put plates with and without UV filter on two different altitudes: 1095 m. a. s. l. and 1504 m. a. s. l. at the sunny side of Kobariški Stol. The experiment took place during entire growing season. We collected samples in June, August and September. The ground temperature was monitored from first to last sampling. In the laboratory, measurements of chlorophylls (a and b), carotenoids, anthocyanins, UV-A and UV-B absorbing compounds were carried out. Also, the measurements of leaf reflectance and transmittance spectra were performed from 280 to 710 nm. We used one-way ANOVA to examine effect of different temperatures and UV radiation levels on plants and we also examined statistically significant differences. The results were compared within one sampling. We examined the effect of altitude and UV radiation level and their interaction on individual biochemical and optical properties of chosen plant with two-way ANOVA. There was no effect of observed environmental factors on content of UV absorbing compounds, which implies plant’s adaptation to UV radiation. There was no effect of temperature and UV radiation level on chlorophyll a and b, carotenoids and anthocyanins content in August, but there was some effect on those pigments in September, which implies early senescence. UV radiation interacts with other environmental factors therefore plants, exposed to different environmental factors on two different altitudes respond differently on UV radiation.

• Keywords: biochemical properties, ecology, Saxifraga hostii, optical properties, UV-B radiation