Odziv vodnih testnih organizmov na prisotnost motilcev endokrinega sistema

Kaučič, Rožle (author), Toman, Mihael J. (mentor)

V pričujoči raziskavi smo proučevali vpliv dveh motilcev endokrinega sistema (MES): fenola bisfenol-A (BPA) in umetnega estrogena etinilestradiol (EE2), ki ju je moč, zaradi narave njune uporabe, pogosto najti v okolju. Njune učinke smo proučevali na vodnih bolhah vrste Daphnia magna, in sicer z uporabo dveh metod za ugotavljanje strupenosti: 1) test akutne strupenosti po ISO 6341:2012 in 2) test kronične strupenosti po OECD Guideline no. 211. Pri slednjih smo poleg učinkov BPA in EE2 posamično preverjali tudi učinke njunih mešanic. Za akutne teste z BPA smo izračunali 24 h EC50 in 48 h EC50. Ker pri testiranih koncentracijah EE2 ni bilo učinkov, zanj teh vrednosti nismo izračunali. Pri kroničnih testih smo poleg števila gibljivih juvenilnih osebkov na samico opazovali še vpliv na pogostost levitev in velikost samic ob koncu testa. Ugotovili smo signifikantne učinke koncentracije BPA 10 mg/L na število juvenilnih osebkov na samico in na končno velikosti le-teh, tako pri testih s posamičnimi snovmi kot tudi v mešanicah. Signifikanten učinek na levitev samic se je pokazal le pri testu z mešanico 10 mg/L BPA in 1 μg/L EE2. Ob nižji koncentraciji BPA (1 mg/L) učinki niso bili signifikantni, kot tudi ne pri testih z EE2 posamično. Ugotovili smo, da MES spreminjajo reprodukcijski potencial in zmanjšujejo reprodukcijsko sposobnost teh testnih vodnih organizmov.

• Language: Slovenian
• Keywords: motilci endokrinega sistema, bisfenol-A, etinilestradiol, ekotoksikologija, Daphnia magna
• Work type: Master's thesis/paper
• Organization: BF - Biotechnical Faculty
• Year: 2019
• PID: 20.500.12556/RUL-111978
• COBISS.SI-ID: 5328719
• Publication date in RUL: 18.10.2019

Secondary language

• Language: English
• Title: Responses of aquatic test organisms to the presence of endocrine disruptors

Abstract

The aim of this study was to look into the effects of the phenol bisphenol A (BPA) and the artificial estrogen ethinyl-estradiol (EE2), two endocrine disrupting compounds (EDCs), which are obiquitous in the environment, as a consequence of their usage. We examined their effects on the water flea Daphnia magna, using two methods for toxicity-testing: 1) an acute toxicity test as per ISO 6341:2012 and 2) a chronic toxicity test as per OECD Guideline no. 211. For the later we also tested the effects of mixtures of these compounds. We calculated the 24 h EC50 and 48 h EC50 values for the acute toxicity tests for BPA, which was not possible for EE2, because of a lack of quantifiable effects. In our chronic tests, we looked into the effects of our test-compounds on the number of mobile juveniles per female, the molting-frequency of the later and their size at the end of the tests. We found significant effects of tests with BPA at 10 mg/L (individualy and in mixtures) on the number of mobile juveniles per female and on the end-of-test size of the females, but not on their molting-frequency, with the exception of a mixture with 10 mg/L BPA and 1 μg/L EE2. Tests with a BPA concentration of 1 mg/L individualy and in mixtures did not show any significant effects, which also holds true for tests with EE2 individualy. We concluded that EDCs impact the reproductive potential and lower the reproduction-abilty of the aquatic organisms used in this study.

• Keywords: Endocrine Disrupting Compounds, Bisphenol A, Ethinyl-Estradiol, Ecotoxicology, Daphnia magna