The use of antibiotics has recently become widespread, so they often occur in the environment. In the case of chronic exposure, bacteria can develop resistance to antibiotics, which means that antibiotics are no longer effective against various bacteria. It is therefore necessary to explore new antibiotics for the treatment of various diseases. The effects of antibiotics on different organisms are not well known. Various organisms may be used for testing of toxicity of antibiotics.
The aim of this master's thesis is to assess the acute impact of antibiotics on water flea and brine shrimp, the cultivation of water flea and the determination of optimal conditions, and the testing and comparison of sensitivity. The tested antibiotics included tiamulin, levofloxacin, amoxicillin and clarithromycin. The cultivation and handling of brine shrimp is easier, but their sensitivity was very low. The antibiotics did not have any effect on brine shrimp up to a concentration of 250 mg/L. Cultivating and handling water fleas requires a lot of time and skills, and the organisms are significantly more sensitive. The results of acute tests have shown that water flea are primarily sensitive to the antibiotic tiamulin. Antibiotics found in the environment are mostly present in concentrations of up to a few μg/L; it is therefore assumed that the tested antibiotics do not have a direct acute effect on these organisms.
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