Stabilnost alfa-amilaze v različnih organizmih

Debevc, Janja (author), Lah, Jurij (mentor)

Primerjala sem stabilnostne krivulje, ki predstavljajo temperaturno odvisnost Gibbsove proste energije razvitja psihrofilnih, mezofilnih in termofilnih α-amilaz. Objavljeni rezultati kažejo, da je stabilnost največja pri termofilni α-amilazi in najmanjša pri psihrofilni α-amilazi. Razvidne so tudi različne temperature, pri katerih se razvijejo tako imenovane temperature taljenja. Najvišja je pri termofilni α-amilazi in najnižja pri psihrofilni α-amilazi.

• Language: Slovenian
• Keywords: α-amilaza, psihrofil, mezofil, termofil
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2019
• PID: 20.500.12556/RUL-111239
• COBISS.SI-ID: 1538437571
• Publication date in RUL: 26.09.2019

Secondary language

• Language: English
• Title: Stability of alpha-amylase in different organisms

Abstract

Stability curves representing the temperature dependence of the Gibbs free energy of the unfolding of psychophilic, mesophilic and thermophilic α-amylases are compared. The published results indicate that the stability is highest in the case of thermophilic α-amylase and lowest for psychophilic α-amylase. The unfolding transition temperatures, the so-called melting temperatures, are also evident. Melting temperature is highest for thermophilic α-amylase and lowest for psychophilic α-amylase.

• Keywords: α-amylase, psihrophile, mesophile, thermophile