Fungi from the genus Verticillium are known to be one of the most harmful plant pathogenic organisms. The genus consists of species that can infect a broad spectrum of plants, many of which are agriculturally relevant crops. In Slovenia a great amout of harm is caused by two species, V. nonalfalfae and V. dahliae. By infecting the hop plant, they can cause a mild or lethal form of a disease, called verticillium wilt. In order to control and potentially even prevent the disease from spreading, the development of suitable diagnostic methods for rapid detection and identification is crucial. The aim of our study was to develop the LAMP method, which can enable a rapid and reliable detection of species of the genus Verticillium and the pathotype PG2. Due to the genus' heterogeneity, we analised a series of isolates via PCR primers specific for the amplicifation of different regions from lethal region of PG2 isolate, by which we wanted to emphasize the difficulties of development of the suitable method and designe of specific primers for molecular diagnostics of the genus Verticillium.
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