Signalne poti v glioblastomskih celicah ob prisotnosti kanabidiola v kombinaciji s kemoterapijo

Lavrič, Luka (author), Lah Turnšek, Tamara (mentor)

Glioblastom (GB) predstavlja najbolj agresivni možganski tumor, ki se predvidoma razvije iz astrocitov. Slaba prognoza bolezni, tudi ob konvencionalni obliki zdravljenja kot je operativni poseg, obsevanje in kemoterapija ne preprečuje napredovanja bolezni. Sočasna (adjuvantna) terapija z naravnimi učinkovinami je način zdravljenja, kjer velikokrat ne poznamo signalnih poti preko katerih te učinkujejo. V nalogi smo raziskali vpliv naravnega kanabinoida, kanabidiola (CBD) samega in v kombinaciji s kemoterapevtikom temozolomidom (TMZ) na signalno pot, ki jo posreduje proteinska kinaza 1/2 (ERK1/2), kar vpliva na proliferacijo, migracijo, invazijo in apoptozo glioblastomskih celic. Ugotovili smo, da se je pri GB celicah U87 izražanje aktivirane fosforilirane oblike ERK proteina, pri tretiranju s CBD zmanjšalo na 43 %, pri CBD v kombinaciji s TMZ pa na 20 %. Na GB U373 celice tretiranje s samim CBD in v kombinaciji s TMZ, ni imelo vpliva.

• Language: Slovenian
• Keywords: glioblastom multiforme, kanabidiol, kemoterapevtik
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2019
• PID: 20.500.12556/RUL-109853
• COBISS.SI-ID: 1538395843
• Publication date in RUL: 09.09.2019

Secondary language

• Language: English
• Title: Signaling pathways in glioblastoma cells in the presence of cannabidiol in combination with chemotherapy

Abstract

Glioblastoma (GB) represents the most aggressive brain tumor, which presumably develops from astrocytes. Ceonventional treatment such as surgery, radiation and chemotherapy, does not enhance poor prognosis and prevent the progression of the disease. Concomitant (adjuvant) therapy with natural substances is rather common way of treatment, where the signaling pathways is often unknown. In this thesis, we have investigated the influence of the natural cannabinoid cannabidiol (CBD) alone and in combination with chemotherapeutic temozolomide (TMZ) on signaling pathway that includes protein kinase ERK1/2, affecing proliferation, migration, invasion and apoptosis of glioblastoma cells. We found that the activated, phosphorilated ERK protein, in GB U87 cells, was reduced down to 43 % after treatment with CBD and to 20 % after treatment with CBD in combination with TMZ. CBD alone or in combination with TMZ did not have any impact on GB U373 cells.

• Keywords: glioblastoma multiforme, cannabidiol, chemoterapeutic