Določanje kovinskih ionov z ICP/OES v medvrstnih križancih bezga

Murovec, Amadej (author), Kolar, Mitja (mentor)

Namen diplomskega dela je bil določiti vsebnost izbranih kovin v koreninah medvrstnih križancev bezga. Pred izvedbo analiz na ICP/OES je bilo treba trdne vzorce pretvoriti v tekočo obliko, ki je primerna za analizo. To sem storil s kislinskimi razklopi vzorcev. Kislinski razklop vzorcev sem izvajal ob prisotnosti HNO3, H2SO4 in H2O2. Tako pripravljeni vzorci so bili razredčeni z 1% HNO3 in shranjeni v 50-mL viale. Sledila je analiza z ICP/OES in za primer magnezija tudi AAS. Pred začetkom merjenja kovin v vzorcih sem izvedel umeritveno krivuljo z uporabo ICP-večelementne standardne raztopine VI (Merck). Vzorcem sem iz umeritvenih krivulj določil koncentracije kovin, v primeru magnezija in kalija pa sem moral vzorce tudi razredčiti. Koncentracije kovin sem interpretiral in določil linearnost merilnega območja.

• Language: Slovenian
• Keywords: bezeg, kovine, kislinski razklopi, induktivno sklopljena plazma - emisijska optična spektroskopija, atomska absorpcijska spektroskopija.
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2019
• PID: 20.500.12556/RUL-109667
• COBISS.SI-ID: 1538327235
• Publication date in RUL: 06.09.2019

Secondary language

• Language: English

Abstract

The purpose of my thesis was to determine concentrations of certain metals in the roots of interconnected crosses of elderberry. Before the ICP/OES analysis, I had to convert solid samples into a liquid form suitable for analysis. I have done this using the acid digestion technique. For this technique I used HNO3, H2SO4 and H2O2. The samples thus prepared were diluted with 1 % HNO3 and were kept in 50 mL vials. The samples were ready for the ICP/OES analysis and in the case of magnesium the AAS analysis was used too. Before the analysis of the samples of elderberry roots I performed the calibration, using a multistandard solution VI (Merck). I have determined concentrations of metals in samples from the calibration curve. For magnesium and potassium, the dilution was needed. I have interpreted the concentrations of metals and determined the linearity of the measuring zone.

• Keywords: elderberry, metals, acid digestion, inductively coupled plasma - optical emission spectrometry, atomic absorption spectroscopy.