Genetic diversity of deformed wing virus from Apis mellifera carnica (Hymenoptera:Apidae) and varroa mite (Mesostigmata: Varroidae)

Jamnikar Ciglenečki, Urška (author), Pislak, Metka (author), Toplak, Ivan (author)

Deformed wing virus (DWV) is one of the most widespread viruses that infect honey bee colonies. The route of infection is directly through contaminated food, feces, and air, or indirectly through the varroa mite, which acts as a vector. Positive DWV samples were obtained from Carniolan honey bee (Apis mellifera carnica) colonies and of varroa mites from the whole territory of Slovenia during a survey between 2007 and 2014. Nucleotide sequences of 471 nucleotides for the L protein gene and 573 nucleotides for the helicase gene were compared. High genetic diversity was observed among these Slovenian Carniolan honey bee DWV field samples, as well as with almost all the strains previously found in other countries in Europe. Phylogenetic analyses in two regions of the viral genome show that several of the DWV strains obtained from honey bees and varroa are genetically very closely related, confirming the important role of varroa in the transmission of DWV. Identification of closely related sequences also confirmed that the same strains of DWV have been successfully transmitted between various honey bee colonies and apiaries. It has also been established that simultaneous infection, in one apiary, of honey bees with two or more different strains of DWV is quite frequent. This is phylogenetic study that compares honey bee and varroa DWV strains from Carniolan honeybees.

• Language: English
• Keywords: bees, deformed wing virus - genetics, varroidae, phylogeny
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Publisher: Oxford University Press
• Year: 2019
• Number of pages: Str. 11-19
• Numbering: Vol. 112, no. 1
• PID: 20.500.12556/RUL-108882
• UDC: 638.15:578.52:575.8
• ISSN on article: 0022-0493
• DOI: 10.1093/jee/toy312
• COBISS.SI-ID: 4686458
• Publication date in RUL: 30.07.2019

Record is a part of a journal

• Title: Journal of economic entomology
• Shortened title: J. econ. entomol.
• Publisher: Entomological Society of America
• ISSN: 0022-0493
• COBISS.SI-ID: 6401031

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 30.07.2019

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0092
• Name: Zdravje živali, okolje in varna hrana