Pomen določanja 8-hidroksideoksigvanozina in 8-izoprostana v urinu otrok s spektrom avtističnih motenj

Mastinšek, Marisa (author), Osredkar, Joško (mentor)

Oksidativni stres vpliva na patogenezo številnih psihiatričnih motenj, saj so možgani bolj dovzetni za oksidativne poškodbe kot drugi organi. Otroci s spektrom avtističnih motenj imajo znižan nivo antioksidantov in s tem zmanjšano sposobnost za boj proti oksidativnemu stresu. Za te otroke je značilen kompleksen vedenjski fenotip in primanjkljaj v socialnih in kognitivnih funkcijah. V okviru naloge smo ovrednotili koncentracije 8-hidroksidegvanozina in 8-izoprostana, ki sta znana označevalca oksidativnega stresa. 8-hidroksidegvanozin je pokazatelj poškodbe DNA, 8-izoprostan pa poškodbe lipidov. Testirali smo skupino otrok z spektrom avtističnih motenj in kontrolno skupino otrok. Koncentracije označevalcev smo določili s kompetitivnim encimsko imunskim testom in rezultate statistično ovrednotili. Otroke s spektrom avtističnih motenj sta sestavljali dve podskupini, in sicer otroci z avtizmom in otroci z Aspergerjevim sindromom. Preverili smo, če med posameznimi skupinami obstaja statistično značilna razlika glede koncentracij 8-hidroksidegvanozina in 8-izoprostana. Pogledali smo koncentracije 8-izoprostana pri posameznih skupinah. Rezultati so pokazali, da imajo otroci s spektrom avtističnih motenj statistično značilno višje koncentracije od kontrolne skupine. Primerjava otrok z avtizmom in z Aspergerjevim sindromom je pokazala značilno višjo produkcijo 8-izoprostana pri otrocih z Aspergerjevim sindromom. Pri skupini otrok z avtizmom in kontrolni skupini otrok pa nismo uspeli dokazati razlik v koncentracijah 8-izoprostana. Zanimala nas je tudi koncentracija 8-hidroksidegvanozina pri posameznih skupinah. Rezultati so pokazali, da med skupinama otrok s spektrom avtističnih motenj in kontrolno skupino ni statistično značilnih razlik. Prav tako nismo uspeli dokazati razlik med avtizmom in Aspergerjevim sindromom ter pri primerjavi le-teh s kontrolno skupino. Zanimala nas je razlika v koncentracijah označevalcev glede na spol in ugotovili smo, da ni korelacije. Otroci s spektrom avtističnih motenj imajo blago, zmerno ali težjo obliko primanjkljajev. Pri primerjavi posameznih oblik smo ugotovili, da ima blaga oblika statistično značilno višjo produkcijo 8-hidroksidegvanozina in 8-izoprostana od drugih dveh. Med zmerno in težjo obliko primanjkljajev pa nismo uspeli dokazati razlik v produkciji označevalcev oksidativnega stresa.

• Language: Slovenian
• Keywords: spekter avtističnih motenj, oksidativni stres, 8-hidroksidegvanozin, 8-izoprostan
• Work type: Master's thesis/paper
• Organization: FFA - Faculty of Pharmacy
• Year: 2019
• PID: 20.500.12556/RUL-108633
• Publication date in RUL: 10.07.2019

Secondary language

• Language: English
• Title: The importance of 8-hydroxydeoxyguanosine and 8-isoprostane determination in urine of children with autistic spectrum disorders

Abstract

Oxidative stress affects the pathogenesis of many psychiatric disorders because the brain is more susceptible to oxidative damage than other organs. Children with autism spectrum disorders have a reduced level of antioxidants and therefore reduced ability to fight oxidative stress. These children are characterized by a complex behavioural phenotype and a deficiency in social and cognitive functions. Our aim was to evaluate the concentrations of 8-hydroxydegvanosine and 8-isoprostane both known as markers of oxidative stress. 8-hydroxydegvanosine is an indicator of DNA damage while 8-isoprosthene shows damage to lipids. We tested a group of children with autism spectrum disorders and a control group of children. Concentrations of both markers were determined by a competitive enzyme immunoassay and the results were statistically evaluated. Children with autism spectrum disorders were composed of two subgroups, namely children with autism and children with Asperger syndrome. We have examined whether there is a significant difference between the individual groups in terms of concentrations of 8-hydroxydegvanosine and 8-isoprostane. We looked at the concentrations of 8-isoprostane in individual groups. The results showed that children with autism spectrum disorders have significantly higher concentrations than the control group. A comparison of children with autism and Asperger syndrome showed a significantly higher production of 8-isoprostane in children with the Asperger syndrome. However, in the group of children with autism and control groups, we were unable to prove differences in concentrations of 8-isoprostane. We were also interested in the concentration of 8-hydroxydegvanosine in individual groups. The results showed that there is no significant difference between the group of children with autism spectrum disorders and the control group. We also failed to demonstrate differences between autism and Asperger syndrome and when comparing both of them with the control group. We were interested in the difference in the concentration of markers by gender and we found that there is no correlation. Children with autism spectrum disorders have a mild, moderate or severe form of deficits. When comparing individual forms, we found that the mild form has significantly higher production of 8-hydroxydegvanosine and 8-isoprostane than the other two. However, we didn’t prove differences between moderate and severe deficits.

• Keywords: autism spectrum disorder, oxidative stress, 8-hydroxydegvanosine, 8-isoprostane